NSP-SA-NHS 

NSP-SA-NHS is an acridinium ester that can be used for chemiluminescent immunoassay. A rapid and sensitive chemiluminescent immunoassay of Bisphenol A (BPA) with NSP-SA-NHS-labeled has been developed^[1].

Guidelines (The following is our recommended experimental protocol. This protocol is for reference only, and specific operations should be adjusted according to your actual needs).
NSP-SA-NHS labeling^[1]
(1) Dissolve 1 mg BSA in 0.3 mL PBS (solution A), and dissolve 0.2 mg NSP-SA-NHS in 40 μL DMF (solution B).
(2) Add solution B to solution A, mix at 18°C and 130 rpm, and protect from light for 12 h.
(3) Dialyze against 500 mL distilled water at 4°C for 2 d, replacing the distilled water twice during this period (solution C).
(4) Slowly add solution B into solution C, mix at 18°C and 130 rpm protected from light for 24 h, and then dialyze against distilled water for 48 h. During this period, replace the distilled water four times.
(5) Dilute the conjugate to an appropriate concentration with distilled water and analyze using a UV spectrophotometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

681.73

C₃₂H₃₁N₃O₁₀S₂

199293-83-9

Solid

Light yellow to yellow

O=S(CCC[N+]1=C2C=CC=CC2=C(C(N(CCCC(ON3C(CCC3=O)=O)=O)S(=O)(C4=CC=C(C)C=C4)=O)=O)C5=C1C=CC=C5)([O-])=O

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Fan Fan Yang, et al. A Rapid and Sensitive Chemiluminescent Immunoassay of Bisphenol a with NSP-SA-NHS-Labeled. Applied Mechanics & Materials, 2014, 707:7-11.