1. PI3K/Akt/mTOR
  2. PDK-1

MP7 

Cat. No.: HY-14440 Purity: 99.36%
Handling Instructions

MP7 is a phosphoinositide-dependent kinase-1 (PDK1) inhibitor.

For research use only. We do not sell to patients.

MP7 Chemical Structure

MP7 Chemical Structure

CAS No. : 1001409-50-2

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 182 In-stock
Estimated Time of Arrival: December 31
5 mg USD 160 In-stock
Estimated Time of Arrival: December 31
10 mg USD 295 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1110 In-stock
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100 mg USD 1680 In-stock
Estimated Time of Arrival: December 31
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Related Small Molecules:

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

MP7 is a phosphoinositide-dependent kinase-1 (PDK1) inhibitor.

IC50 & Target

PDK1[1]

In Vitro

Cell counting of U87MG-derived glioma stem cells (GSCs) confirms that Alisertib and, to a minor extent, MP7 are able to decrease the number of viable cells. When combined together, GSC viability is further reduced with respect to single-treated cells. As observed in U87MG cells, when used at the highest concentrations (i.e., 1.5 μM Alisertib and 2.5 μM MP7), a significant enhancement in the number of dead cells is evidenced. Following 72 h treatment, MP7 alone does not show a significant inhibition of glioblastoma multiforme (GBM) proliferation. MP7 has been shown to have only minimal effects on monolayer cell growth in several cancer cell lines, with IC50 values in the micromolar range[1].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9361 mL 9.6805 mL 19.3611 mL
5 mM 0.3872 mL 1.9361 mL 3.8722 mL
10 mM 0.1936 mL 0.9681 mL 1.9361 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

The human GBM cells (i.e., U87MG, U343MG, or ANGM-CSS) or the respective GSCs are seeded and incubated for the indicated times with the indicated concentrations of SA16 (1 nM to 100 μM), MP7 (2.5 nM, 25 nM, 250 nM and 2.5 μM), or Alisertib. When indicated, cells are treated with MP7 and Alisertib in combination. To verify GSC chemoresistance, U87MG or GSCs are incubated with 50 μM TMZ for 72 h. For the long-term treatment of cells, NSC or complete medium containing drugs is replaced every 3 days. Cell proliferation is determined using the MTS assay: the dehydrogenase activity in active mitochondria reduces MTS to the soluble formazan product, whose absorbance at 490 nm is measured with an automated plate reader. The mean background from each test condition is subtracted, and the data are expressed as the percentage of untreated cells (control). IC50 values are derived from the sigmoid dose-response curve. The percentage of inhibition is calculated as 100% minus the percentage of cell proliferation[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

516.50

Formula

C₂₈H₂₂F₂N₄O₄

CAS No.

1001409-50-2

SMILES

O=C(C1=CC=CN(CC2=CC=C(F)C(F)=C2)C1=O)N[[email protected]](C3=CC=CC=C3)COC4=CC=C(N5)C(NC5=O)=C4

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
MP7
Cat. No.:
HY-14440
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MP7

Cat. No.: HY-14440