1. Biochemical Assay Reagents
  2. PIPES

PIPES (Synonyms: 1,4-Piperazinediethanesulfonic acid)

Cat. No.: HY-D0875 Purity: ≥98.0%
Handling Instructions

PIPES (1,4-Piperazinediethanesulfonic acid) is an important component of PIPES buffer agent used in biochemistry.

For research use only. We do not sell to patients.

PIPES Chemical Structure

PIPES Chemical Structure

CAS No. : 5625-37-6

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Free Sample (0.5-1 mg)   Apply Now  
10 mM * 1 mL in Water USD 55 In-stock
Estimated Time of Arrival: December 31
500 mg USD 50 In-stock
Estimated Time of Arrival: December 31
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PIPES (1,4-Piperazinediethanesulfonic acid) is an important component of PIPES buffer agent used in biochemistry[1].

In Vitro

To prepare the pH PIPES buffer, 173 g of 1,4-piperazinediethanesulfonic acid are dissolved into 1 L of deionized water. The pH of the PIPES buffer is adjusted to 6.8 by adding pellets of sodium hydroxide.
PIPES buffer can be used to PIPES buffer, it can prevent the glutaraldehyde fixation induced lipid loss and artifacts[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight









Room temperature in continental US; may vary elsewhere.

Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 5 mg/mL (16.54 mM; ultrasonic and adjust pH to 7 with NaOH)

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3072 mL 16.5360 mL 33.0721 mL
5 mM 0.6614 mL 3.3072 mL 6.6144 mL
10 mM 0.3307 mL 1.6536 mL 3.3072 mL
*Please refer to the solubility information to select the appropriate solvent.
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  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2


PIPES1,4-Piperazinediethanesulfonic acidOthersbuffer PIPEScell culturesample prepareglutaraldehyde fixativeInhibitorinhibitorinhibit

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