Cat. No.: HY-N0816

Polyphyllin VI

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

Polyphyllin VI, an active saponin, possess anti-cancer activities. Polyphyllin VI induces G2/M cell cycle arrest and triggers apoptosis. Polyphyllin VI induces caspase-1-mediated pyroptosis via the induction of ROS/NF-κB/NLRP3/GSDMD signal axis in non-small cell lung cancer.

For research use only. We do not sell to patients.

Purity: 98.49%
CAS No.: 55916-51-3
Formula: C₃₉H₆₂O₁₃
Molecular Weight:738.90

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Polyphyllin VI Chemical Structure

CAS No.: 55916-51-3

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (6)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	IC₅₀	5.2 μg/mL Compound: 5	Cytotoxicity against human HL60 cells by MTT assay Cytotoxicity against human HL60 cells by MTT assay	[PMID: 11000027]

In Vitro

Polyphyllin VI (0-16 μM; 48 h) significantly decreases the viability of A549, NCI-H1299 and HepaRG cells in a dose-dependent manner^[1]^[2].
Polyphyllin VI (0.5-2 μM-1 μM; 24 h) significantly increases the percentage of A549, and NCI-H1299 cells in the G2/M stage in a dose-dependent manner^[1].
Polyphyllin VI (0-12 μM-1 μM; 24 h) arrests HepaRG cells at S stage ^[2].
Polyphyllin VI (0-6 μM-1 μM; 24 h) activates the NLRP3 inflammasome^[3].
Polyphyllin VI (0-6 μM-1 μM; 24 h) induces pyroptosis via ROS/NF-κB pathway om A549 and NCI-H1299 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]^[3]

Cell Line:

A549, NCI-H1299, and HepaRG cells
Concentration:

0 μM, 2.5 μM, 5.0 μM, 7.5 μM, 10 μM, 12.5 μM (A549, NCI-H1299), 0 μM, 2 μM, 4.0 μM, 6.0 μM, 8.0 μM, 10.0 μM, 12.0 μM, 16.0 μM (HepaRG)
Incubation Time:

48 h (A549, NCI-H1299), 24 h and 48 h (HepaRG)
Result:

Showed the IC₅₀ value in NCI-H1299 cells after 48h treatment was 1.87±0.09 μM, 1.59±0.12 μM in A549^[1].
Demonstrated the reduction of HepaRG cell viability ranged from 88.90% to 1.07% after 24 h, and from 79.06% to 0.71% after 48 h^[2].

Cell Viability Assay^[1]

Cell Line:

A549, NCI-H1299 cells
Concentration:

0.5 μM, 1.0 μM, 2.0 μM
Incubation Time:

24 h
Result:

Showed those of A549 cells in G2/M phase were 25.14%±3.31%, 28.40%±4.63%, and 42.66%±1.30%, and NCI-H1299 cells were 27.99%±4.68%, 30.24%±3.61% and 38.51%±5.10% after treatment with 0.5, 1, and 2μM for 24 h, respectively.

Cell Cycle Analysis^[2]

Cell Line:

HepaRG cells
Concentration:

0 μM, 2.0 μM, 4.0 μM, 6.0 μM, 8.0 μM, 12.0 μM
Incubation Time:

24 h
Result:

Resulted the ratios of cells in the S and G0/G1 phase changed from 23.62%±0.14% to 34.01%±0.32%, 66.88%±1.15% to 54.00%±0.71%, respectively.

Western Blot Analysis^[3]

Cell Line:

A549, NCI-H1299 cells
Concentration:

0 μM, 3 μM, 4 μM, 5 μM, 6 μM
Incubation Time:

24 h
Result:

Resulted dose-dependently increasing the protein expression of NLRP3 and ASC, and the cleaved form of caspase-1, IL-1β, IL-18 and GSDMD in A549 and NCI-H1299 cells.

Immunofluorescence^[3]

Cell Line:

A549, NCI-H1299 cells
Concentration:

0 μM, 3 μM, 4 μM, 5 μM, 6 μM
Incubation Time:

24 h
Result:

Resulted the expression of caspase-1 was significantly inhibited by N-acetyl-L-cysteine (NAC) in Polyphyllin VI -treated A549 and NCI-H1299 cells.

In Vivo

Polyphyllin VI (2-4 mg/kg; i.p.; five times a week for 4 weeks) inhibits the growth of lung cancer tumor xenografts^[1].
Polyphyllin VI (2.5-10 mg/kg; i.p.; 10 consecutive days) activates NLRP3 inflammasome in A549-bearing athymic nude mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

A549 tumor xenografts subcutaneously inoculated into the right flank of the nude mice^[1]
Dosage:

2 mg/kg, 3 mg/kg, 4 mg/kg
Administration:

Intraperitoneal Injection (i.p.)
Result:

Resulted the reduction of tumor volume to 25.63%, 41.71%, and 40.41%, respectively, after 2 mg/kg, 3 mg/kg and 4 mg/kg treatment.

Animal Model:

A549 tumor xenografts subcutaneously inoculated into the right flank of the nude mice^[3]
Dosage:

2.5 mg/kg, 5 mg/kg, 10 mg/kg
Administration:

Intraperitoneal Injection (i.p.)
Result:

Showed the expression of NLRP3, caspase-1, IL-1β and GSDMD was increasing in a dose manner.

Chemical Information

CAS No. 55916-51-3
Appearance Solid
Molecular Weight 738.90
Formula C₃₉H₆₂O₁₃
Color White to off-white
SMILES

O[C@]1([C@@]2([H])O[C@]3(CC[C@@H](C)CO3)[C@H]1C)[C@]([C@@]4([H])C2)(CC[C@@]5([H])[C@@]4([H])CC=C6[C@@]5(CC[C@H](O[C@@](O[C@H](CO)[C@@H](O)[C@@H]7O)([H])[C@@H]7O[C@@](O[C@@H](C)[C@H](O)[C@H]8O)([H])[C@@H]8O)C6)C)C
Structure Classification
- Steroids
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (6)

All Cell Proliferation/Viability AssayBio/Physico-chemical AssayFlow Cytometry

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Journal Impact Factor

Cancer Res

Genome-Wide CRISPR Screening Reveals that mTOR Inhibition Initiates Ferritinophagy and Ferroptosis in Head and Neck Cancer. [Abstract]

2025 Jun 6. PMID: 40479615

Polyphyllin VI purchased from MedChemExpress. Usage Cited in: Cancer Res. 2025 Jun 6. [Abstract]

Cell viability of Cal27 treated with INK128 (50 nM) for 72 hours was measured. From left to right, Z-VAD, Necrostatin-1 and Ac-FEID-CMK were used in combination with INK128. Positive controls were Etoposide (1.5 μM) and Polyphyllin VI (PPVI, 5 μM; 72 h). Cell viability was compared between monotherapy and combination therapy (mean ± SD, n = 6).
ACS Nano

Targeted Positron Emission Tomography-Tracked Biomimetic Codelivery Synergistically Amplifies Ferroptosis and Pyroptosis for Inducing Lung Cancer Regression and Anti-PD-L1 Immunotherapy Efficacy. [Abstract]

2024 Nov 12;18(45):31401-31420. PMID: 39475541

Polyphyllin VI purchased from MedChemExpress. Usage Cited in: ACS Nano. 2024 Nov 12;18(45):31401-31420. [Abstract]

Polyphyllin VI (PPVI, 24 h) exhibited IC₅₀ values of 6.41 μM and 16.26 μM in A549 and H1299 cells, respectively.
Dev Cell

Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation. [Abstract]

2025 Apr 18:S1534-5807(25)00206-0. PMID: 40280135

Polyphyllin VI purchased from MedChemExpress. Usage Cited in: Dev Cell. 2025 Apr 18:S1534-5807(25)00206-0. [Abstract]

Measurement of caspase 1 (CASP1) activity in control A549 cells and A549 cells withCCN1 overexpression following treatment with RSL3 (0.5 μM, 24 h) or erastin (10 μM, 24h). Treatment with Polyphyllin VI (2 μM, 24 h) was set as the positive control to induce CASP1 activity.
Int Immunopharmacol

Polyphyllin VI: A promising treatment for prostate cancer bone metastasis. [Abstract]

2024 Nov 26:144:113684. PMID: 39602960
Int Immunopharmacol

Stachyose ameliorates myocardial ischemia-reperfusion injury by inhibiting cardiomyocyte ferroptosis and macrophage pyroptosis. [Abstract]

2024 Oct 8;143(Pt 1):113334. PMID: 39383784
Mediators Inflamm

Downregulated XBP-1 Rescues Cerebral Ischemia/Reperfusion Injury-Induced Pyroptosis via the NLRP3/Caspase-1/GSDMD Axis. [Abstract]

2022 Apr 21;2022:8007078. PMID: 35497095

Polyphyllin VI purchased from MedChemExpress. Usage Cited in: Mediators Inflamm. 2022 Apr 21;2022:8007078. [Abstract]

The HT22 cells were, respectively, pretreated with Z-YVAD-FMK (20 μM) and Polyphyllin VI (4 μM) 30 min before exposing to OGD/R for 24 h. Cell viability was detected in HT22 cells using a CCK-8 assay.

Polyphyllin VI purchased from MedChemExpress. Usage Cited in: Mediators Inflamm. 2022 Apr 21;2022:8007078. [Abstract]

The HT22 cells were, respectively, pretreated with XBP-1 siRNA, 24 h before exposing to OGD/R for 24 h and Z-YVAD-FMK (20 μM) and Polyphyllin VI (4 μM) 30 min before exposing to OGD/R for 24 h. The quantity of necrotic cells and pyroptotic cells were analyzed by Annexin V and PI staining in each group.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (135.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.3534 mL

6.7668 mL

13.5336 mL

5 mM

0.2707 mL

1.3534 mL

2.7067 mL

10 mM

0.1353 mL

0.6767 mL

1.3534 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1 mg/mL (1.35 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 1 mg/mL (1.35 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1 mg/mL (1.35 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (286 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Lin Z, et al. Anti-lung Cancer Effects of Polyphyllin VI and VII Potentially Correlate with Apoptosis In Vitro and In Vivo. Phytother Res. 2015 Oct;29(10):1568-76. [Content Brief]

[2]. Liu Y, et al. Molecular Mechanisms of Apoptosis in HepaRG Cell Line Induced by Polyphyllin VI via the Fas Death Pathway and Mitochondrial-Dependent Pathway. Toxins (Basel). 2018 May 15;10(5). pii: E201. [Content Brief]

[3]. Jin-Feng Teng, et al. Polyphyllin VI Induces Caspase-1-Mediated Pyroptosis via the Induction of ROS/NF-κB/NLRP3/GSDMD Signal Axis in Non-Small Cell Lung Cancer. Cancers (Basel). 2020 Jan 13;12(1):193. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.3534 mL	6.7668 mL	13.5336 mL	33.8341 mL
	5 mM	0.2707 mL	1.3534 mL	2.7067 mL	6.7668 mL
	10 mM	0.1353 mL	0.6767 mL	1.3534 mL	3.3834 mL
	15 mM	0.0902 mL	0.4511 mL	0.9022 mL	2.2556 mL
	20 mM	0.0677 mL	0.3383 mL	0.6767 mL	1.6917 mL
	25 mM	0.0541 mL	0.2707 mL	0.5413 mL	1.3534 mL
	30 mM	0.0451 mL	0.2256 mL	0.4511 mL	1.1278 mL
	40 mM	0.0338 mL	0.1692 mL	0.3383 mL	0.8459 mL
	50 mM	0.0271 mL	0.1353 mL	0.2707 mL	0.6767 mL
	60 mM	0.0226 mL	0.1128 mL	0.2256 mL	0.5639 mL
	80 mM	0.0169 mL	0.0846 mL	0.1692 mL	0.4229 mL
	100 mM	0.0135 mL	0.0677 mL	0.1353 mL	0.3383 mL

Keywords

Polyphyllin VI

6 Cited Publications

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Polyphyllin VI

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Publications Citing Use of MedChemExpress (MCE) Polyphyllin VI

Biological Activity

Chemical Information

Publications (6)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Polyphyllin VI Related Classifications

Cat. No.: HY-N0816