ACAT1 Protein, Human (His)

The ACAT1 protein is a key enzyme in mitochondrial beta-oxidation and plays a crucial role in breaking down fatty acids into acetyl-CoA. In the final step, ACAT1 catalyzes the thiolytic cleavage of 3-oxoacyl-CoA to generate acetyl-CoA and shortened fatty acyl-CoA. ACAT1 Protein Protein, Human (His) is the recombinant human-derived ACAT1 protein, expressed by E. coli , with N-His labeled tag.

The ACAT1 protein is a pivotal enzyme in the mitochondrial beta-oxidation pathway, a vital aerobic process responsible for breaking down fatty acids into acetyl-CoA. Operating in the last step of this pathway, ACAT1 utilizes free coenzyme A (CoA) to catalyze the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs, generating acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. This reversible enzymatic activity extends to the condensation of two acetyl-CoA molecules into acetoacetyl-CoA, underscoring ACAT1's essential role in ketone body metabolism. The multifaceted functions of ACAT1 illuminate its significance in regulating key metabolic processes, shedding light on its integral role in maintaining cellular energy balance and homeostasis.

Human

E. coli

N-10*His

P24752-1 (V34-L427)

38  [NCBI]

Full Length of Isoform-1 Mature Protein

VSKPTLKEVVIVSATRTPIGSFLGSLSLLPATKLGSIAIQGAIEKAGIPKEEVKEAYMGNVLQGGEGQAPTRQAVLGAGLPISTPCTTINKVCASGMKAIMMASQSLMCGHQDVMVAGGMESMSNVPYVMNRGSTPYGGVKLEDLIVKDGLTDVYNKIHMGSCAENTAKKLNIARNEQDAYAINSYTRSKAAWEAGKFGNEVIPVTVTVKGQPDVVVKEDEEYKRVDFSKVPKLKTVFQKENGTVTAANASTLNDGAAALVLMTADAAKRLNVTPLARIVAFADAAVEPIDFPIAPVYAASMVLKDVGLKKEDIAMWEVNEAFSLVVLANIKMLEIDPQKVNINGGAVSLGHPIGMSGARIVGHLTHALKQGEYGLASICNGGGGASAMLIQKL

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of Tris-based buffer, 50% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
2.Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.