ACAT1 Protein, Human (His)
Based on 1 publication(s) in Google Scholar
The ACAT1 protein is a key enzyme in mitochondrial beta-oxidation and plays a crucial role in breaking down fatty acids into acetyl-CoA. In the final step, ACAT1 catalyzes the thiolytic cleavage of 3-oxoacyl-CoA to generate acetyl-CoA and shortened fatty acyl-CoA. ACAT1 Protein Protein, Human (His) is the recombinant human-derived ACAT1 protein, expressed by E. coli , with N-His labeled tag.
- Species: Human
- Source: E. coli
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The ACAT1 protein is a key enzyme in mitochondrial beta-oxidation and plays a crucial role in breaking down fatty acids into acetyl-CoA. In the final step, ACAT1 catalyzes the thiolytic cleavage of 3-oxoacyl-CoA to generate acetyl-CoA and shortened fatty acyl-CoA. ACAT1 Protein Protein, Human (His) is the recombinant human-derived ACAT1 protein, expressed by E. coli , with N-His labeled tag.
Background
The ACAT1 protein is a pivotal enzyme in the mitochondrial beta-oxidation pathway, a vital aerobic process responsible for breaking down fatty acids into acetyl-CoA. Operating in the last step of this pathway, ACAT1 utilizes free coenzyme A (CoA) to catalyze the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs, generating acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. This reversible enzymatic activity extends to the condensation of two acetyl-CoA molecules into acetoacetyl-CoA, underscoring ACAT1's essential role in ketone body metabolism. The multifaceted functions of ACAT1 illuminate its significance in regulating key metabolic processes, shedding light on its integral role in maintaining cellular energy balance and homeostasis.
Publications (1)
-
Journal Impact Factor
-
Most Recent
Technical Parameters
-
Species Human
-
Source E. coli
-
Tag N-10*His
-
Accession
P24752-1 (V34-L427)
-
Molecular Construction
-
N-term
-
His
-
ACAT1 (V34-L427)
Accession # P24752 -
C-term
-
-
Protein Length
Full Length of Isoform-1
-
Synonyms
ACAT1; Acetyl-Coenzyme A Acetyltransferase 1; Prev. ACAT; MAT; Acetyl-CoA Acetyltransferase, Mitochondrial; T2; Acetoacetyl-CoA Thiolase; Mitochondrial Acetoacetyl-CoA Thiolase; THIL; Testicular Tissue Protein Li 198; Acetyl-CoA Acetyltransferase 1; Aceto
-
AA Sequence
VSKPTLKEVVIVSATRTPIGSFLGSLSLLPATKLGSIAIQGAIEKAGIPKEEVKEAYMGNVLQGGEGQAPTRQAVLGAGLPISTPCTTINKVCASGMKAIMMASQSLMCGHQDVMVAGGMESMSNVPYVMNRGSTPYGGVKLEDLIVKDGLTDVYNKIHMGSCAENTAKKLNIARNEQDAYAINSYTRSKAAWEAGKFGNEVIPVTVTVKGQPDVVVKEDEEYKRVDFSKVPKLKTVFQKENGTVTAANASTLNDGAAALVLMTADAAKRLNVTPLARIVAFADAAVEPIDFPIAPVYAASMVLKDVGLKKEDIAMWEVNEAFSLVVLANIKMLEIDPQKVNINGGAVSLGHPIGMSGARIVGHLTHALKQGEYGLASICNGGGGASAMLIQKL
-
Predicted Molecular Mass
45 kDa
-
Molecular Weight
Approximately 41 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of Tris-based buffer, 50% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
2.Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (237 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
