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Alkaline Phosphatase/ALPL Protein, Mouse (HEK293, His)

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Alkaline Phosphatase/ALPL Protein is a phosphatase whose physiological function is to dephosphorylate compounds. ALPL is also a membrane-bound glycosylated enzyme that catalyzes the hydrolysis of phosphate esters at alkaline pH. Mice that lack ALPL show symptoms of osteomalacia, softening of the bones. Moreover, ALPL stimulates mineralization mainly through modulation of the balance between inorganic phosphate (Pi) and inorganic pyrophosphate (PPi) and also has a role in cardiovascular remodelling. Alkaline Phosphatase/ALPL Protein, Mouse (HEK293, His) is the recombinant mouse-derived Alkaline Phosphatase/ALPL protein, expressed by HEK293 , with C-8*His labeled tag.

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  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Alkaline Phosphatase/ALPL Protein, Mouse (HEK293, His)
  • Alkaline Phosphatase/ALPL Protein, Mouse (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

Alkaline Phosphatase/ALPL Protein is a phosphatase whose physiological function is to dephosphorylate compounds. ALPL is also a membrane-bound glycosylated enzyme that catalyzes the hydrolysis of phosphate esters at alkaline pH. Mice that lack ALPL show symptoms of osteomalacia, softening of the bones. Moreover, ALPL stimulates mineralization mainly through modulation of the balance between inorganic phosphate (Pi) and inorganic pyrophosphate (PPi) and also has a role in cardiovascular remodelling. Alkaline Phosphatase/ALPL Protein, Mouse (HEK293, His) is the recombinant mouse-derived Alkaline Phosphatase/ALPL protein, expressed by HEK293 , with C-8*His labeled tag.

Background

Alkaline phosphatase (ALPL) is a phosphatase whose physiological function is to dephosphorylate compounds. ALPL is also a membrane-bound glycosylated enzyme that catalyzes the hydrolysis of phosphate esters at alkaline pH. The mature peptide maintains the ratio of inorganic phosphate to inorganic pyrophosphate required for bone mineralization. Mice that lack ALPL show symptoms of osteomalacia, softening of the bones. In humans, mutations in this gene are associated with hypophosphatasia, an inherited metabolic bone disease in which deficiency of this enzyme inhibits bone mineralization leading to skeletal defects. ALPL is highly expressed in the cells of mineralized tissue and plays a critical function in the formation of hard tissue. And ALPL increases inorganic phosphate local rates and facilitates mineralization as well as reduces the extracellular pyrophosphate concentration, an inhibitor of mineral formation. Moreover, ALPL stimulates mineralization mainly through modulation of the balance between inorganic phosphate (Pi) and inorganic pyrophosphate (PPi) and also has a role in cardiovascular remodelling[1][2][3].

Verified Bioactivity

Measured by its ability to cleave 25 μM fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP) that at room temperature for 5 minutes. The specific activity is 60917.96 pmol/min/µg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-8*His
  • Accession

    B7XGA6/BAH03518.1 (F18-S502)

  • Gene ID
    11647  [NCBI]
  • Molecular Construction
    • N-term
    • ALPL (F18-S502)
      Accession # B7XGA6/BAH03518.1
    • 8*His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    ALPL; Alkaline Phosphatase, Liver/Bone/Kidney; Prev. HOPS; TNS-ALP; TNSALP; Liver/Bone/Kidney-Type Alkaline Phosphatase; Alkaline Phosphatase, Tissue-Nonspecific Isozyme; Tissue-Nonspecific ALP; Alkaline Phosphatase Liver/Bone/Kidney Isozyme; Alkaline Pho

  • AA Sequence

    FVPEKERDPSYWRQQAQETLKNALKLQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNTGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERTRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIKDIDVIMGGGRKYMYPKNRTDVEYELDEKARGTRLDGLDLISIWKSFKPRHKHSHYVWNRTELLALDPSRVDYLLGLFEPGDMQYELNRNNLTDPSLSEMVEVALQILTKNPKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDQAIGKAGAMTSQKDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGYKVVDGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYASCIGANLDHCAWAGS

  • Molecular Weight

    Approximately 55 kDa

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
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MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Alkaline Phosphatase/ALPL Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Alkaline Phosphatase/ALPL Protein, Mouse (HEK293, His)
  • Alkaline Phosphatase/ALPL Protein, Mouse (HEK293, His) Product Image