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  5. Aminopeptidase N/APN Protein, Mouse (HEK293, His)

Aminopeptidase N/APN Protein, Mouse (HEK293, His)

Cat. No.: HY-P70051
COA Handling Instructions

Aminopeptidase N/APN proteins act as aminopeptidases with broad specificity, assisting in the final stages of peptide digestion and processing of various peptides, including the hormones angiotensin III/IV and neuropeptides. Aminopeptidase N/APN Protein, Mouse (HEK293, His) is the recombinant mouse-derived Aminopeptidase N/APN protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Aminopeptidase N/APN Protein, Mouse (HEK293, His) is 898 a.a., with molecular weight of 110-130 kDa.

For research use only. We do not sell to patients.

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Description

Aminopeptidase N/APN proteins act as aminopeptidases with broad specificity, assisting in the final stages of peptide digestion and processing of various peptides, including the hormones angiotensin III/IV and neuropeptides. Aminopeptidase N/APN Protein, Mouse (HEK293, His) is the recombinant mouse-derived Aminopeptidase N/APN protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Aminopeptidase N/APN Protein, Mouse (HEK293, His) is 898 a.a., with molecular weight of 110-130 kDa.

Background

Aminopeptidase N/APN Protein exhibits broad specificity as an aminopeptidase, contributing to the final stages of peptide digestion arising from the hydrolysis of proteins by gastric and pancreatic proteases. Beyond its digestive role, it participates in the processing of various peptides, including peptide hormones like angiotensin III and IV, neuropeptides, and chemokines. Additionally, it is implicated in the cleavage of peptides bound to major histocompatibility complex class II molecules on antigen-presenting cells. Aminopeptidase N/APN may play a role in angiogenesis, cholesterol crystallization promotion, and potentially acts as a binding partner for amino acid transporter SLC6A19, thereby regulating its activity and influencing amino acid transport.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Mouse

Source

HEK293

Tag

C-6*His

Accession

P97449 (K69-S966)

Gene ID

16790  [NCBI]

Molecular Construction
N-term
APN (K69-S966)
Accession # P97449
6*His
C-term
Synonyms
rMuAminopeptidase N/APN, His; Aminopeptidase N; ANPEP; AP-M; APN; AP-N; CD13 antigen; CD13; CD13APN; PEPN; PEPNhAPN
AA Sequence

KPWNQYRLPKTLIPDSYRVILRPYLTPNNQGLYIFQGNSTVRFTCNQTTDVIIIHSKKLNYTLKGNHRVVLRTLDGTPAPNIDKTELVERTEYLVVHLQGSLVEGRQYEMDSQFQGELADDLAGFYRSEYMEGDVKKVVATTQMQAADARKSFPCFDEPAMKAMFNITLIYPNNLIALSNMLPKESKPYPEDPSCTMTEFHSTPKMSTYLLAYIVSEFKNISSVSANGVQIGIWARPSAIDEGQGDYALNVTGPILNFFAQHYNTSYPLPKSDQIALPDFNAGAMENWGLVTYRESSLVFDSQSSSISNKERVVTVIAHELAHQWFGNLVTVAWWNDLWLNEGFASYVEYLGADYAEPTWNLKDLMVLNDVYRVMAVDALASSHPLSSPADEIKTPDQIMELFDSITYSKGASVIRMLSSFLTEDLFKKGLSSYLHTYQYSNTVYLDLWEHLQKAVNQQTAVQPPATVRTIMDRWILQMGFPVITVNTNTGEISQKHFLLDSKSNVTRPSEFNYIWIAPIPFLKSGQEDHYWLDVEKNQSAKFQTSSNEWILLNINVTGYYLVNYDENNWKKLQNQLQTDLSVIPVINRAQIIHDSFNLASAKMIPITLALDNTLFLVKEAEYMPWQAALSSLNYFTLMFDRSEVYGPMKRYLKKQVTPLFFYFQNRTNNWVNRPPTLMEQYNEINAISTACSSGLKECRDLVVELYSQWMKNPNNNTIHPNLRSTVYCNAIAFGGEEEWNFAWEQFRNATLVNEADKLRSALACSKDVWILNRYLSYTLNPDYIRKQDTTSTIISIASNVAGHPLVWDFVRSNWKKLFENYGGGSFSFANLIQGVTRRFSSEFELQQLEQFKADNSATGFGTGTRALEQALEKTRANIDWVKENKDAVFKWFTENSS

Molecular Weight

110-130 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

N/A

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Aminopeptidase N/APN Protein, Mouse (HEK293, His)
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HY-P70051
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