Animal-Free BMP-7 Protein, Human (His)

BMP-7 protein is an important member of the TGF-β superfamily and is critical in embryogenesis, hematopoiesis, neurogenesis and bone morphogenesis. It activates canonical BMP signaling through ACVR1 and ACVR2A, phosphorylates and activates ACVR1, which in turn phosphorylates SMAD1/5/8 for target gene transcriptional regulation. Animal-Free BMP-7 Protein, Human (His) is the recombinant human-derived animal-FreeBMP-7 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free BMP-7 Protein, Human (His) is 117 a.a., with molecular weight of ~14.00 kDa.

BMP-7 Protein, a vital member of the TGF-beta superfamily, plays a crucial role in various biological processes, encompassing embryogenesis, hematopoiesis, neurogenesis, and skeletal morphogenesis. It initiates the canonical BMP signaling cascade by binding to the type I receptor ACVR1 and the type II receptor ACVR2A, leading to the phosphorylation and activation of ACVR1. Subsequently, ACVR1 phosphorylates SMAD1/5/8, which modulate target gene transcription as activators and repressors in the nucleus. In specific functions, such as growth cone collapse in developing spinal neurons and monocyte chemotaxis, BMP-7 utilizes BMPR2 as an additional type II receptor. Beyond canonical pathways, BMP-7 signals through non-canonical routes, such as the P38 MAP kinase signaling cascade, to promote brown adipocyte differentiation by activating target genes, including members of the SOX family of transcription factors. BMP-7 further regulates the expression of HAMP, with this process being restrained by its interaction with ERFE. This homodimeric protein forms disulfide-linked complexes and interacts with various proteins, including SOSTDC1, TWSG1, FBN1, FBN2, ACVR1, ACVR2A, NOG, SCUBE3, and ERFE, each contributing to its diverse functional repertoire.

Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <0.65 μg/mL.

Human

E. coli

C-His

P18075 (M315-H431)

655  [NCBI]

MANVAENSSSDQRQACKKHELYVSFRDLGWQDWIIAPEGYAAYYCEGECAFPLNSYMNATNHAIVQTLVHFINPETVPKPCCAPTQLNAISVLYFDDSSNVILKKYRNMVVRACGCH

Approximately 14.00 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a solution containing 20 mM sodium citrate, 0.2 MNaCl, pH 3.5.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Sun R, et al. Expression of BMP-7 in cervical cancer and inhibition of epithelial‑mesenchymal transition by BMP-7 knockdown in HeLa cells. Int J Mol Med. 2020 May;45(5):1417-1424.  [Content Brief]

  [2]. Miyazawa K, et al. Regulation of TGF-β Family Signaling by Inhibitory Smads. Cold Spring Harb Perspect Biol. 2017 Mar 1;9(3):a022095.  [Content Brief]

  [3]. Dorai H, et al. Bone morphogenetic protein-7 (osteogenic protein-1) inhibits smooth muscle cell proliferation and stimulates the expression of markers that are characteristic of SMC phenotype in vitro. J Cell Physiol. 2000 Jul;184(1):37-45.  [Content Brief]

  [4]. Mathew S, et al. Function and effect of bone morphogenetic protein-7 in kidney bone and the bone-vascular links in chronic kidney disease. Eur J Clin Invest. 2006 Aug;36 Suppl 2:43-50.  [Content Brief]

  [5]. Yang P, et al. The role of bone morphogenetic protein signaling in vascular calcification. Bone. 2020 Dec;141:115542.  [Content Brief]

  [6]. Xu RH, et al. BMP4 initiates human embryonic stem cell differentiation to trophoblast. Nat Biotechnol. 2002 Dec;20(12):1261-4.  [Content Brief]

  [7]. Beck HN, et al. Bone morphogenetic protein-5 (BMP-5) promotes dendritic growth in cultured sympathetic neurons. BMC Neurosci. 2001;2:12.  [Content Brief]