Animal-Free BMP-7 Protein, Human (His)
Based on 1 Customer Validation
BMP-7 protein is an important member of the TGF-β superfamily and is critical in embryogenesis, hematopoiesis, neurogenesis and bone morphogenesis. It activates canonical BMP signaling through ACVR1 and ACVR2A, phosphorylates and activates ACVR1, which in turn phosphorylates SMAD1/5/8 for target gene transcriptional regulation. Animal-Free BMP-7 Protein, Human (His) is the recombinant human-derived animal-FreeBMP-7 protein, expressed by E. coli , with C-His labeled tag. This product is for cell culture use only.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
BMP-7 protein is an important member of the TGF-β superfamily and is critical in embryogenesis, hematopoiesis, neurogenesis and bone morphogenesis. It activates canonical BMP signaling through ACVR1 and ACVR2A, phosphorylates and activates ACVR1, which in turn phosphorylates SMAD1/5/8 for target gene transcriptional regulation. Animal-Free BMP-7 Protein, Human (His) is the recombinant human-derived animal-FreeBMP-7 protein, expressed by E. coli , with C-His labeled tag. This product is for cell culture use only.
Background
BMP-7 Protein, a vital member of the TGF-beta superfamily, plays a crucial role in various biological processes, encompassing embryogenesis, hematopoiesis, neurogenesis, and skeletal morphogenesis. It initiates the canonical BMP signaling cascade by binding to the type I receptor ACVR1 and the type II receptor ACVR2A, leading to the phosphorylation and activation of ACVR1. Subsequently, ACVR1 phosphorylates SMAD1/5/8, which modulate target gene transcription as activators and repressors in the nucleus. In specific functions, such as growth cone collapse in developing spinal neurons and monocyte chemotaxis, BMP-7 utilizes BMPR2 as an additional type II receptor. Beyond canonical pathways, BMP-7 signals through non-canonical routes, such as the P38 MAP kinase signaling cascade, to promote brown adipocyte differentiation by activating target genes, including members of the SOX family of transcription factors. BMP-7 further regulates the expression of HAMP, with this process being restrained by its interaction with ERFE. This homodimeric protein forms disulfide-linked complexes and interacts with various proteins, including SOSTDC1, TWSG1, FBN1, FBN2, ACVR1, ACVR2A, NOG, SCUBE3, and ERFE, each contributing to its diverse functional repertoire.
Verified Bioactivity
Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is <0.65 μg/mL.
Technical Parameters
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Species Human
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Source E. coli
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Tag C-6*His
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Accession
P18075 (M315-H431)
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Molecular Construction
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N-term
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BMP-7 (M315-H431)
Accession # P18075 -
6*His
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C-term
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Protein Length
Partial
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Synonyms
BMP7; Eptotermin Alfa; Bone Morphogenetic Protein 7; BMP-7; Osteogenic Protein 1; OP1; OP-1
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AA Sequence
MANVAENSSSDQRQACKKHELYVSFRDLGWQDWIIAPEGYAAYYCEGECAFPLNSYMNATNHAIVQTLVHFINPETVPKPCCAPTQLNAISVLYFDDSSNVILKKYRNMVVRACGCH
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Predicted Molecular Mass
14 kDa
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Molecular Weight
Approximately 12 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 20 mM sodium citrate, 0.2 M NaCl, pH 3.5, trehalose.
<0.1 EU per 1 μg of the protein by the LAL method.
It is recommended to reconstitute to a concentration of 100-200 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Sun R, et al. Expression of BMP-7 in cervical cancer and inhibition of epithelial‑mesenchymal transition by BMP-7 knockdown in HeLa cells. Int J Mol Med. 2020 May;45(5):1417-1424. [Content Brief]
[2]. Miyazawa K, et al. Regulation of TGF-β Family Signaling by Inhibitory Smads. Cold Spring Harb Perspect Biol. 2017 Mar 1;9(3):a022095. [Content Brief]
[3]. Dorai H, et al. Bone morphogenetic protein-7 (osteogenic protein-1) inhibits smooth muscle cell proliferation and stimulates the expression of markers that are characteristic of SMC phenotype in vitro. J Cell Physiol. 2000 Jul;184(1):37-45. [Content Brief]
[4]. Mathew S, et al. Function and effect of bone morphogenetic protein-7 in kidney bone and the bone-vascular links in chronic kidney disease. Eur J Clin Invest. 2006 Aug;36 Suppl 2:43-50. [Content Brief]
[5]. Yang P, et al. The role of bone morphogenetic protein signaling in vascular calcification. Bone. 2020 Dec;141:115542. [Content Brief]
[6]. Xu RH, et al. BMP4 initiates human embryonic stem cell differentiation to trophoblast. Nat Biotechnol. 2002 Dec;20(12):1261-4. [Content Brief]
[7]. Beck HN, et al. Bone morphogenetic protein-5 (BMP-5) promotes dendritic growth in cultured sympathetic neurons. BMC Neurosci. 2001;2:12. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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