Animal-Free Galectin-2/LGALS2 Protein, Human (His)

The Galectin-2 (LGALS2) protein binds with affinity to β-galactopyranoside and forms homodimers, indicating a basic functional organization. Although the physiological role is unclear, β-galactoside binding of LGALS2 suggests a possible involvement in recognition of specific carbohydrates. Animal-Free Galectin-2/LGALS2 Protein, Human (His) is the recombinant human-derived animal-FreeGalectin-2/LGALS2 protein, expressed by E. coli , with N-His labeled tag. This product is for cell culture use only.

The Galectin-2 (LGALS2) protein demonstrates an affinity for binding beta-galactosides, yet its precise physiological function remains unclear. Structurally, it forms homodimers, indicating a fundamental organization in its functional state. While the specific role of LGALS2 in cellular processes is yet to be elucidated, its ability to bind to beta-galactosides suggests potential involvement in recognizing and interacting with specific carbohydrate structures. The homodimeric arrangement further implies a cooperative mechanism in its functional activity, emphasizing the need for further exploration to unveil the intricate biological significance of LGALS2 in cellular contexts and molecular interactions.

Measured by its ability to agglutinate human red blood cells. The ED₅₀ for this effect is <20 μg/mL

Human

E. coli

N-6*His

P05162 (T2-E132)

3957  [NCBI]

Partial

TGELEVKNMDMKPGSTLKITGSIADGTDGFVINLGQGTDKLNLHFNPRFSESTIVCNSLDGSNWGQEQREDHLCFSPGSEVKFTVTFESDKFKVKLPDGHELTFPNRLGHSHLSYLSVRGGFNMSSFKLKE

Approximately 15.5 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is recommended to reconstitute to a concentration of 100-200 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.