Animal-Free Galectin-8/LGALS8 Protein, Human (His)

Galectin-8/LGALS8 Protein, a vital enzyme in glycosylation, serves as a beta-1,3-N-acetylglucosaminyltransferase, synthesizing poly-N-acetyllactosamine. Crucial for modifying glycoproteins and glycolipids, Galectin-8/LGALS8 catalyzes N-acetylglucosamine transfer onto acceptor molecules, exhibiting specific activity for type 2 oligosaccharides. Its role in poly-N-acetyllactosamine synthesis emphasizes its significance in modulating cellular interactions, impacting various processes like cell adhesion, signaling, and recognition events through glycan structure alterations. Animal-Free Galectin-8/LGALS8 Protein, Human (His) is the recombinant human-derived animal-FreeGalectin-8/LGALS8 protein, expressed by E. coli, with N-His labeled tag. The total length of Animal-Free Galectin-8/LGALS8 Protein, Human (His) is 316 a.a., with molecular weight of ~36.6 kDa.

B3GNT4, a key enzyme in glycosylation processes, functions as a beta-1,3-N-acetylglucosaminyltransferase responsible for synthesizing poly-N-acetyllactosamine. This enzyme plays a crucial role in the modification of glycoproteins and glycolipids by catalyzing the transfer of N-acetylglucosamine residues onto acceptor molecules. Notably, B3GNT4 exhibits specific activity for type 2 oligosaccharides, contributing to the diversification and complexity of glycan structures. The synthesis of poly-N-acetyllactosamine by B3GNT4 underscores its significance in modulating cellular interactions, as alterations in glycan structures can impact various biological processes, including cell adhesion, signaling, and recognition events.

Measured by its ability to agglutinate human red blood cells. The ED₅₀ for this effect is <8 μg/mL.

Human

E. coli

N-His

O00214 (M2-W317)

3964  [NCBI]

MLSLNNLQNIIYNPVIPFVGTIPDQLDPGTLIVIRGHVPSDADRFQVDLQNGSSMKPRADVAFHFNPRFKRAGCIVCNTLINEKWGREEITYDTPFKREKSFEIVIMVLKDKFQVAVNGKHTLLYGHRIGPEKIDTLGIYGKVNIHSIGFSFSSDLQSTQASSLELTEISRENVPKSGTPQLRLPFAARLNTPMGPGRTVVVKGEVNANAKSFNVDLLAGKSKDIALHLNPRLNIKAFVRNSFLQESWGEEERNITSFPFSPGMYFEMIIYCDVREFKVAVNGVHSLEYKHRFKELSSIDTLEINGDIHLLEVRSW

Approximately 36.6 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a solution containing 1X PBS, pH 7.4.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.