1. Recombinant Proteins
  2. Cytokines and Growth Factors Animal-free Recombinant Proteins
  3. Interleukin & Receptors
  4. IL-1RA
  5. Animal-Free IL-1RA/IL-1RN Protein, Human (His)

Animal-Free IL-1RA/IL-1RN Protein, Human (His)

Cat. No.: HY-P700110AF
COA Handling Instructions

IL-1RA/IL-1RN proteins are potent anti-inflammatory antagonists in the interleukin 1 family, specifically targeting the proinflammatory cytokines IL1B and IL1A. It protects against immune dysregulation and, crucially, prevents IL1 from triggering uncontrolled systemic inflammation in response to various innate stimulants, including pathogens. Animal-Free IL-1RA/IL-1RN Protein, Human (His) is the recombinant human-derived animal-FreeIL-1RA/IL-1RN protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-1RA/IL-1RN Protein, Human (His) is 152 a.a., with molecular weight of ~18.07 kDa.

Animal-free recombinant proteins offer high consistency and stability, whithout using or contacting of any animal-derived materials and reagents.

For research use only. We do not sell to patients.

Protein Expression Service

Size Price Stock Quantity
Free Sample   Apply now
2 μg $55 In-stock
10 μg $90 In-stock
50 μg $190 In-stock
100 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

IL-1RA/IL-1RN proteins are potent anti-inflammatory antagonists in the interleukin 1 family, specifically targeting the proinflammatory cytokines IL1B and IL1A. It protects against immune dysregulation and, crucially, prevents IL1 from triggering uncontrolled systemic inflammation in response to various innate stimulants, including pathogens. Animal-Free IL-1RA/IL-1RN Protein, Human (His) is the recombinant human-derived animal-FreeIL-1RA/IL-1RN protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-1RA/IL-1RN Protein, Human (His) is 152 a.a., with molecular weight of ~18.07 kDa.

Background

The IL-1RA/IL-1RN protein stands as a potent anti-inflammatory antagonist within the interleukin-1 family, specifically targeting proinflammatory cytokines like interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Serving as a safeguard against immune dysregulation, this protein is instrumental in preventing uncontrolled systemic inflammation triggered by IL1 in response to various innate stimulatory agents, including pathogens. Its nature underscores its utility in research and therapeutic applications, providing a reliable and ethical tool for studying and mitigating inflammatory processes without reliance on animal-derived materials.

Biological Activity

Measure by its ability to inhibit IL-1 alpha-dependent proliferation in D10.G4.1 cells. The ED50 for this effect is <50 ng/mL.

Species

Human

Source

E. coli

Tag

C-His

Accession

P18510-1 (R26-E177)

Gene ID
Molecular Construction
N-term
IL-1RA (R26-E177)
Accession # P18510
His
C-term
Synonyms
ICIL-1RA; IRAP; IL-1RN
AA Sequence

MRPSGRKSSKMQAFRIWDVNQKTFYLRNNQLVAGYLQGPNVNLEEKIDVVPIEPHALFLGIHGGKMCLSCVKSGDETRLQLEAVNITDLSENRKQDKRFAFIRSDSGPTTSFESAACPGWFLCTAMEADQPVSLTNMPDEGVMVTKFYFQEDE

Molecular Weight

Approximately 18.07 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a solution containing 1X PBS, pH 7.4.

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Animal-Free IL-1RA/IL-1RN Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Animal-Free IL-1RA/IL-1RN Protein, Human (His)
Cat. No.:
HY-P700110AF
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