Animal-Free IL-21 Protein, Human (His)

IL-21 protein is an immunomodulatory cytokine that promotes the transition from innate immunity to adaptive immunity. It induces B cells to produce IgG(1) and IgG(3), harnessing an effective antibody response to fight viral infections. Animal-Free IL-21 Protein, Human (His) is the recombinant human-derived animal-FreeIL-21 protein, expressed by E. coli , with C-His labeled tag. This product is for cell culture use only.

IL-21 Protein, a cytokine with immunoregulatory activity, serves as a pivotal mediator in orchestrating the interplay between innate and adaptive immunity. Notably, it induces the production of IgG(1) and IgG(3) in B-cells, emphasizing its role in shaping humoral immune responses. Additionally, IL-21 is intricately involved in the generation and maintenance of T follicular helper (Tfh) cells, playing a crucial part in the formation of germinal centers—an essential process in adaptive immunity. Collaborating with IL6, it contributes to the early generation of Tfh cells, proving critical for mounting an effective antibody response during acute viral infections. Furthermore, IL-21 may play a role in the proliferation and maturation of natural killer (NK) cells, particularly in synergy with IL15. Its regulatory influence extends to mature B- and T-cells, modulating their proliferation in response to activating stimuli. In concert with IL15 and IL18, IL-21 stimulates interferon gamma production in T-cells and NK cells, highlighting its multifaceted impact on immune responses. During T-cell-mediated immune responses, IL-21 exhibits the potential to inhibit dendritic cells (DC) activation and maturation, adding another layer to its regulatory repertoire.

Measure by its ability to enhance IFN gamma secretion in NK-92 cells. The ED₅₀ for this effect is <10 ng/mL.

Human

E. coli

C-6*His

Q9HBE4-1 (Q32-S162)

59067  [NCBI]

Partial

QDRHMIRMRQLIDIVDQLKNYVNDLVPEFLPAPEDVETNCEWSAFSCFQKAQLKSANTGNNERIINVSIKKLKRKPPSTNAGRRQKHRLTCPSCDSYEKKPPKEFLERFKSLLQKMIHQHLSSRTHGSEDS

Approximately 20 kDa, based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 8.0, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is recommended to reconstitute to a concentration of 100-200 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.