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  4. B7-1/CD80
  5. B7-1/CD80 Protein, Rat (HEK293, Fc)

B7-1/CD80 Protein, Rat (HEK293, Fc)

Cat. No.: HY-P74398
Handling Instructions

B7-1/CD80, a type I membrane protein, is a member of the B7 family, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. CD80 is also a transmembrane glycoprotein and a member of the Ig superfamily. CD80 has a crucial role in modulating T-cell immune function as a checkpoint protein at the immunological synapse. Its binding to CD28 or CTLA-4 receptors is integral to inducing T-cell proliferation and cytokine production. B7-1/CD80 Protein, Rat (HEK293, Fc) is the recombinant rat-derived B7-1/CD80 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of B7-1/CD80 Protein, Rat (HEK293, Fc) is 248 a.a., with molecular weight of ~51 kDa.

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Description

B7-1/CD80, a type I membrane protein, is a member of the B7 family, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. CD80 is also a transmembrane glycoprotein and a member of the Ig superfamily. CD80 has a crucial role in modulating T-cell immune function as a checkpoint protein at the immunological synapse. Its binding to CD28 or CTLA-4 receptors is integral to inducing T-cell proliferation and cytokine production. B7-1/CD80 Protein, Rat (HEK293, Fc) is the recombinant rat-derived B7-1/CD80 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of B7-1/CD80 Protein, Rat (HEK293, Fc) is 248 a.a., with molecular weight of ~51 kDa.

Background

B7-1/CD80, a type I membrane protein, is a member of the B7 family, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. CD80 is also a transmembrane glycoprotein and a member of the Ig superfamily. CD80 can be found on the surface of various immune cells, including B-cells, monocytes, or T-cells, but most typically at antigen-presenting cells (APCs) such as dendritic cells. CD80 has a crucial role in modulating T-cell immune function as a checkpoint protein at the immunological synapse. CD80 binds to CD28 and CTLA-4 with lower affinity and fast binding kinetics, allowing for quick interactions between the communicating cells. These interactions result in an important costimulatory signal in the immunological synapse between antigen-presenting cells, B-cells, dendritic cells and T-cells that result in T and B-cell activation, proliferation and differentiation. Moreover, the interaction of CD80 with CD28, together with TCR and MHC interaction, results in activation of nuclear factor‐κB (NF-ⲕB), mitogen‐activated protein kinase (MAPK), and the calcium‐calcineurin pathway. These changes initiate the production of numerous factors, cytokines, and chemokines by T-cells. CD80, often in tandem with CD86, plays a large and diverse role in regulating both the adaptive and the innate immune system. In addition to interactions with CD28 and CTLA-4, CD80 is also thought to interact with a separate ligand on Natural Killer cells, triggering the Natural Killer cell-mediated cell death of the CD80 carrier[1][2][3].

Species

Rat

Source

HEK293

Tag

C-hFc

Accession

AAB60503 (M1-Q248)

Gene ID

25408  [NCBI]

Molecular Construction
N-term
B7-1 (M1-Q248)
Accession # AAB60503
hFc
C-term
Synonyms
T-lymphocyte activation antigen CD80; Activation B7-1 antigen; BB1; B7; CD80; CD28LG; LAB7
Molecular Weight

Approximately 51 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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B7-1/CD80 Protein, Rat (HEK293, Fc)
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HY-P74398
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