BSSP-4 Protein, Human (HEK293, His)
BSSP-4 Protein is notable for its specific preference in cleaving the synthetic substrate H-D-Leu-Thr-Arg-pNA over tosyl-Gly-Pro-Arg-pNA. This enzymatic selectivity indicates BSSP-4's potential modulation of specific peptide sequences, emphasizing its relevance in biological processes. BSSP-4 Protein, Human (HEK293, His) is the recombinant human-derived BSSP-4 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Biological Activity
Description
BSSP-4 Protein is notable for its specific preference in cleaving the synthetic substrate H-D-Leu-Thr-Arg-pNA over tosyl-Gly-Pro-Arg-pNA. This enzymatic selectivity indicates BSSP-4's potential modulation of specific peptide sequences, emphasizing its relevance in biological processes. BSSP-4 Protein, Human (HEK293, His) is the recombinant human-derived BSSP-4 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
The BSSP-4 protein takes center stage as it exhibits a distinct preference for cleaving the synthetic substrate H-D-Leu-Thr-Arg-pNA in comparison to tosyl-Gly-Pro-Arg-pNA. This enzymatic specificity highlights BSSP-4's selectivity in substrate recognition and cleavage, showcasing its potential role in modulating specific peptide sequences. The preference for H-D-Leu-Thr-Arg-pNA over tosyl-Gly-Pro-Arg-pNA suggests a substrate specificity that could be relevant to the protein's functional role in biological processes.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
Q9GZN4 (A33-S317)
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Molecular Construction
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N-term
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BSSP-4 (A33-S317)
Accession # Q9GZN4 -
6*His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
PRSS22; Serine Protease 26; Serine Protease 22; Tryptase Epsilon; BSSP-4; Protease, Serine S1 Family Member 22; Brain-Specific Serine Protease 4; Prosemin; HBSSP-4; PRSS26; SP001LA; BSSP4; Protease, Serine 22
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AA Sequence
ARIPVPPACGKPQQLNRVVGGEDSTDSEWPWIVSIQKNGTHHCAGSLLTSRWVITAAHCFKDNLNKPYLFSVLLGAWQLGNPGSRSQKVGVAWVEPHPVYSWKEGACADIALVRLERSIQFSERVLPICLPDASIHLPPNTHCWISGWGSIQDGVPLPHPQTLQKLKVPIIDSEVCSHLYWRGAGQGPITEDMLCAGYLEGERDACLGDSGGPLMCQVDGAWLLAGIISWGEGCAERNRPGVYISLSAHRSWVEKIVQGVQLRGRAQGGGALRAPSQGSGAAARS
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Predicted Molecular Mass
31.6 kDa
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Molecular Weight
Approximately 33-37 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)