C1D Protein, Human (His)

The C1D protein has multifunctionality in cellular processes, recruiting RNA exosome complexes for 5.8S rRNA processing, possibly in conjunction with MPHOSPH6. It activates PRKDC with linear and supercoiled DNA and induces apoptosis through the p53/TP53 pathway. C1D Protein, Human (His) is the recombinant human-derived C1D protein, expressed by E. coli , with N-6*His labeled tag.

C1D, a multifaceted protein, orchestrates various cellular processes with distinct functional implications. It plays a pivotal role in recruiting the RNA exosome complex to pre-rRNA, facilitating the intricate 3'-5' end processing of the 5.8S rRNA, possibly in collaboration with MPHOSPH6. Additionally, C1D exhibits a unique capability to activate PRKDC, not only in the presence of linear DNA but also in the presence of supercoiled DNA. Notably, it can induce apoptosis through a p53/TP53 dependent pathway, showcasing its regulatory role in programmed cell death. Furthermore, C1D may modulate the formation of the TRAX/TSN complex and enhances transcriptional repression by NR1D1 and THRB. As a molecular entity, C1D exists in monomeric and homodimeric forms, interacting with a spectrum of proteins including NR1D1, THRA, THRB, NCOR1, NCOR2, EXOSC10, TSNAX, and RAC3. This intricate network of interactions underscores the diverse functions and regulatory potential of C1D in cellular biology.

Human

E. coli

N-6*His

Q13901 (M1-S141)

10438  [NCBI]

Full Length

MAGEEINEDYPVEIHEYLSAFENSIGAVDEMLKTMMSVSRNELLQKLDPLEQAKVDLVSAYTLNSMFWVYLATQGVNPKEHPVKQELERIRVYMNRVKEITDKKKAGKLDRGAASRFVKNALWEPKSKNASKVANKGKSKS

Approximately 18 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.