NOV/CCN3 Protein, Mouse (HEK293, His)

NOV/CCN3 protein, functioning as an immediate-early participant, plays a pivotal role in a diverse array of cellular processes encompassing proliferation, adhesion, migration, differentiation, and survival. Operating through binding interactions with integrins or membrane receptors such as NOTCH1, NOV/CCN3 emerges as an essential regulator of hematopoietic stem and progenitor cell function. It inhibits myogenic differentiation by activating the Notch-signaling pathway and curtails vascular smooth muscle cells proliferation independently of TGFB1 signaling, influencing the expression of cell-cycle regulators like CDKN2B or CDKN1A. As a ligand for integrins ITGAV:ITGB3 and ITGA5:ITGB1, NOV/CCN3 directly stimulates pro-angiogenic activities, induces angiogenesis, and supports endothelial cell adhesion, migration, and survival. Additionally, it plays roles in cutaneous wound healing, skin fibroblast adhesion, and chemotaxis through interactions with various integrin pairs. Moreover, NOV/CCN3 contributes to bone regeneration, negatively regulating the articular chondrocytic phenotype while repressing endochondral ossification. It also affects pancreatic beta-cell function, acting as a negative regulator by inhibiting beta-cell proliferation and insulin secretion. Functioning as a negative regulator of endothelial pro-inflammatory activation, it reduces monocyte adhesion and exerts anti-inflammatory effects by inhibiting the NF-kappaB signaling pathway. NOV/CCN3 further contributes to the control of inflammatory processes in atherosclerosis and attenuates inflammatory pain through the regulation of IL1B- and TNF-induced MMP9, MMP2, and CCL2 expression, inhibiting MMP9 expression through engagement with ITGB1. It engages in diverse protein interactions, including FBLN1, NOTCH1, GJA1/CX43, ITGA5:ITGB1, ITGAV:ITGB3, ITGAV:ITGB5, and ZDHHC22, potentially leading to CCN3 palmitoylation. The breadth of these interactions underscores the intricate and multifaceted role of NOV/CCN3 in cellular processes and regulatory pathways.