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  2. CD Antigens Fc Receptors
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  4. Fc epsilon RII/CD23
  5. CD23/Fc epsilon RII Protein, Mouse (HEK293, His)

CD23/Fc epsilon RII Protein, Mouse (HEK293, His)

Cat. No.: HY-P74321
COA Handling Instructions

CD23/Fc epsilon RII Protein, a low-affinity receptor for IgE and CR2/CD21, regulates IgE production and B cell differentiation. On B cells, it initiates IgE-dependent antigen uptake and presentation to T cells. On macrophages, IgE binding induces intracellular killing of parasites through the L-Arginine-nitric oxide pathway. CD23/Fc epsilon RII homotrimer interacts with IGHE to regulate IgE homeostasis and with CR2/CD21 for binding. CD23/Fc epsilon RII Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD23/Fc epsilon RII protein, expressed by HEK293 , with N-His labeled tag. The total length of CD23/Fc epsilon RII Protein, Mouse (HEK293, His) is 282 a.a., with molecular weight of 33-43 kDa.

For research use only. We do not sell to patients.

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Description

CD23/Fc epsilon RII Protein, a low-affinity receptor for IgE and CR2/CD21, regulates IgE production and B cell differentiation. On B cells, it initiates IgE-dependent antigen uptake and presentation to T cells. On macrophages, IgE binding induces intracellular killing of parasites through the L-Arginine-nitric oxide pathway. CD23/Fc epsilon RII homotrimer interacts with IGHE to regulate IgE homeostasis and with CR2/CD21 for binding. CD23/Fc epsilon RII Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD23/Fc epsilon RII protein, expressed by HEK293 , with N-His labeled tag. The total length of CD23/Fc epsilon RII Protein, Mouse (HEK293, His) is 282 a.a., with molecular weight of 33-43 kDa.

Background

The CD23/Fc epsilon RII protein serves as a low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21, playing crucial roles in the regulation of IgE production and B cell differentiation. On B cells, it initiates IgE-dependent antigen uptake and presentation to T cells, contributing to immune response modulation. In macrophages, CD23, upon binding IgE and cross-linking with antigens, triggers intracellular killing of parasites through the activation of the L-Arginine-nitric oxide pathway. Existing as a homotrimer, CD23 interacts with IGHE, regulating IgE homeostasis through its C-type lectin domain. Additionally, CD23 interacts with CR2/CD21 through its C-terminus, forming a connection via the Sushi domains 1 and 2. These interactions underscore the multifaceted involvement of CD23 in immunological processes, influencing both B cell function and macrophage-mediated responses.

Biological Activity

Immobilized recombinant mouse CD23 at 5 μg/ml (100 μl/well) can bind Biotinylated Recombinant mouse IgE Protein. The ED50 for this effect is 0.1294 μg/mL.

  • Immobilized recombinant mouse CD23 at 5 μg/ml (100 μl/well) can bind Biotinylated Recombinant mouse IgE Protein. The ED50 for this effect is 0.1294 μg/mL.
Species

Mouse

Source

HEK293

Tag

N-His

Accession

P20693-1 (E50-P331)

Gene ID

14128  [NCBI]

Molecular Construction
N-term
His
CD23 (E50-P331)
Accession # P20693-1
C-term
Synonyms
Low affinity immunoglobulin epsilon Fc receptor; BLAST-2; FCER2; CD23A
Molecular Weight

33-43 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. or 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
CD23/Fc epsilon RII Protein, Mouse (HEK293, His)
Cat. No.:
HY-P74321
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