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  5. CD3E/CD3 epsilon 1-27 peptide Protein, Cynomolgus (Biotinylated, HEK293, Fc-Avi)

CD3E/CD3 epsilon 1-27 peptide Protein, Cynomolgus (Biotinylated, HEK293, Fc-Avi)

Cat. No.: HY-P77608
Handling Instructions

The CD3E/CD3 epsilon 1-27 peptide is critical in the TCR-CD3 complex, transmitting signals during T cell activation. When APC activates the TCR, CD3E undergoes LCK/FYN-mediated phosphorylation together with ITAM, initiating downstream signaling. CD3E/CD3 epsilon 1-27 peptide Protein, Cynomolgus (Biotinylated, HEK293, Fc-Avi) is the recombinant cynomolgus-derived CD3E/CD3 epsilon 1-27 peptide protein, expressed by HEK293 , with C-Avi, C-hFc labeled tag. The total length of CD3E/CD3 epsilon 1-27 peptide Protein, Cynomolgus (Biotinylated, HEK293, Fc-Avi) is 27 a.a., with molecular weight of 40-50 kDa.

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Description

The CD3E/CD3 epsilon 1-27 peptide is critical in the TCR-CD3 complex, transmitting signals during T cell activation. When APC activates the TCR, CD3E undergoes LCK/FYN-mediated phosphorylation together with ITAM, initiating downstream signaling. CD3E/CD3 epsilon 1-27 peptide Protein, Cynomolgus (Biotinylated, HEK293, Fc-Avi) is the recombinant cynomolgus-derived CD3E/CD3 epsilon 1-27 peptide protein, expressed by HEK293 , with C-Avi, C-hFc labeled tag. The total length of CD3E/CD3 epsilon 1-27 peptide Protein, Cynomolgus (Biotinylated, HEK293, Fc-Avi) is 27 a.a., with molecular weight of 40-50 kDa.

Background

The CD3E/CD3 epsilon 1-27 peptide, integral to the TCR-CD3 complex on T-lymphocyte cell surfaces, plays a crucial role in adaptive immune responses. Upon activation by antigen-presenting cells (APCs), the T-cell receptor (TCR) transmits signals across the cell membrane through CD3 chains, including CD3D, CD3E, CD3G, and CD3Z, each containing immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domains. Phosphorylation of these ITAMs by Src family protein tyrosine kinases LCK and FYN, upon TCR engagement, activates downstream signaling pathways. Beyond its role in signal transduction, CD3E is indispensable for correct T-cell development, initiating the assembly of the TCR-CD3 complex by forming heterodimers CD3D/CD3E and CD3G/CD3E. Additionally, CD3E participates in the internalization and cell surface down-regulation of TCR-CD3 complexes through endocytosis sequences in its cytosolic region. The TCR-CD3 complex, comprising CD3D/CD3E and CD3G/CD3E heterodimers, preferentially associates with TCRalpha and TCRbeta, forming trimers. This hexamer then interacts with CD3Z homodimers to complete the TCR-CD3 complex. Alternatively, TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta. The CD3E/CD3 epsilon 1-27 peptide also interacts with CD6, NCK1, and NUMB, with the NUMB interaction being crucial for TCR-CD3 internalization and subsequent degradation.

Species

Cynomolgus

Source

HEK293

Tag

C-Avi;C-hFc

Accession

Q95LI5 (D22-T48)

Gene ID

102133065  [NCBI]

Molecular Construction
N-term
CD3E (D22-T48)
Accession # Q95LI5
hFc-Avi
C-term
Synonyms
CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18
Molecular Weight

40-50 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

CD3E/CD3 epsilon 1-27 peptide Protein, Cynomolgus (Biotinylated, HEK293, Fc-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
CD3E/CD3 epsilon 1-27 peptide Protein, Cynomolgus (Biotinylated, HEK293, Fc-Avi)
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HY-P77608
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