CD6 Protein, Human (HEK293, Fc)

The CD6 protein is a cell adhesion molecule that mediates cell-cell contacts and regulates T cell responses through interaction with ALCAM/CD166. It functions as a costimulatory molecule, promoting T cell activation, proliferation, and contributing to the formation of immune synapses. CD6 Protein, Human (HEK293, Fc) is the recombinant human-derived CD6 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD6 Protein, Human (HEK293, Fc) is 381 a.a., with molecular weight of ~67.3 kDa.

CD6 protein, a pivotal cell adhesion molecule, plays a central role in mediating cell-cell contacts and orchestrating T-cell responses through its interaction with ALCAM/CD166. This interaction is crucial for regulating T-cell activation and proliferation, contributing to the formation and maturation of the immunological synapse. Beyond its role in T-cell responses, CD6 functions as a calcium-dependent pattern receptor, binding and aggregating both Gram-positive and Gram-negative bacteria, with downstream activation of signaling cascades and MAP kinases in response to lipopolysaccharide (LPS) from Gram-negative bacteria. CD6's involvement in the inflammatory response includes mediating the secretion of pro-inflammatory cytokines upon LPS stimulation. CD6 interacts with various proteins, including the TCR/CD3 complex subunit CD3E, LCP2, VAV1, and glycosylated extracellular domains of LGALS1 and LGALS3. Notably, interactions with LGALS1 or LGALS3 inhibit the binding of CD6 to ALCAM, providing further insight into the intricate regulatory mechanisms of CD6-mediated cellular functions.

Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×10⁴ cells/well are added to CD6-Fc coated plates (5 μg/mL and 100 μL/well) in the ppresence of 10 μg/ml PHA, approximately > 50% cells will adhere specifically after 60 minutes at 37°C.

Human

HEK293

C-hFc

P30203 (H18-E398)

923  [NCBI]

MWLFFGITGLLTAALSGHPSPAPPDQLNTSSAESELWEPGERLPVRLTNGSSSCSGTVEVRLEASWEPACGALWDSRAAEAVCRALGCGGAEAASQLAPPTPELPPPPAAGNTSVAANATLAGAPALLCSGAEWRLCEVVEHACRSDGRRARVTCAENRALRLVDGGGACAGRVEMLEHGEWGSVCDDTWDLEDAHVVCRQLGCGWAVQALPGLHFTPGRGPIHRDQVNCSGAEAYLWDCPGLPGQHYCGHKEDAGAVCSEHQSWRLTGGADRCEGQVEVHFRGVWNTVCDSEWYPSEAKVLCQSLGCGTAVERPKGLPHSLSGRMYYSCNGEELTLSNCSWRFNNSNLCSQSLAARVLCSASRSLHNLSTPEVPASVQTVTIESSVTVKIENKESRE

Approximately 67.3 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.