Chitinase-like protein 3/YM1 Protein, Mouse (HEK293, His)

Chitinase-like protein 3/YM1, functioning as a lectin, demonstrates a strong affinity for saccharides containing free amino groups, particularly oligomeric saccharides. It binds to chitin and heparin, displaying weak hexosaminidase activity without chitinase activity. Notably, the protein exhibits chemotactic activity for T-lymphocytes, bone marrow cells, and eosinophils, indicating potential roles in inflammation and allergies. Chitinase-like protein 3/YM1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Chitinase-like protein 3/YM1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Chitinase-like protein 3/YM1 Protein, Mouse (HEK293, His) is 377 a.a., with molecular weight of 41-45 kDa.

The Chitinase-like protein 3/YM1 protein functions as a lectin with a notable affinity for saccharides containing a free amino group, including glucosamine or galactosamine. Its binding affinity is considerably stronger for oligomeric saccharides compared to mono- or disaccharides. Additionally, Chitinase-like protein 3/YM1 binds to chitin and heparin. While it exhibits weak hexosaminidase activity, it does not possess chitinase activity. Beyond its enzymatic functions, the protein displays chemotactic activity for T-lymphocytes, bone marrow cells, and eosinophils, suggesting potential involvement in inflammation and allergy processes.

Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. The ED₅₀ for this effect is 0.9299 μg/mL, corresponding to a specific activity is 1075.3844 units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. The ED₅₀ for this effect is 0.9299 μg/mL, corresponding to a specific activity is 1075.3844 units/mg.

Mouse

HEK293

C-6*His

O35744 (Y22-Y398)

12655  [NCBI]

YQLMCYYTSWAKDRPIEGSFKPGNIDPCLCTHLIYAFAGMQNNEITYTHEQDLRDYEALNGLKDKNTELKTLLAIGGWKFGPAPFSAMVSTPQNRQIFIQSVIRFLRQYNFDGLNLDWQYPGSRGSPPKDKHLFSVLVKEMRKAFEEESVEKDIPRLLLTSTGAGIIDVIKSGYKIPELSQSLDYIQVMTYDLHDPKDGYTGENSPLYKSPYDIGKSADLNVDSIISYWKDHGAASEKLIVGFPAYGHTFILSDPSKTGIGAPTISTGPPGKYTDESGLLAYYEVCTFLNEGATEVWDAPQEVPYAYQGNEWVGYDNVRSFKLKAQWLKDNNLGGAVVWPLDMDDFSGSFCHQRHFPLTSTLKGDLNIHSASCKGPY

41-45 kDa

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  Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.