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  3. Hydrolases (EC 3)
  4. Coagulation Factor VII/F7 Protein, Human (HEK293, His)

Coagulation Factor VII/F7 Protein, Human (HEK293, His)

Cat. No.: HY-P74239
COA Handling Instructions

Coagulation factor VII (F7) initiates the extrinsic pathway of blood coagulation. As a circulating zymogen, F7 is activated to F7a through proteolysis induced by factor Xa, factor XIIa, factor IXa, or thrombin. In the presence of tissue factor and calcium ions, F7a catalyzes limited proteolysis of factor X, converting it to factor Xa. F7a can also convert factor IX to factor IXa, emphasizing its pivotal role in coagulation cascades. Coagulation Factor VII/F7 Protein, Human (HEK293, His) is the recombinant human-derived Coagulation Factor VII/F7 protein, expressed by HEK293 , with C-His labeled tag. The total length of Coagulation Factor VII/F7 Protein, Human (HEK293, His) is 406 a.a., with molecular weight of ~50 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

Coagulation factor VII (F7) initiates the extrinsic pathway of blood coagulation. As a circulating zymogen, F7 is activated to F7a through proteolysis induced by factor Xa, factor XIIa, factor IXa, or thrombin. In the presence of tissue factor and calcium ions, F7a catalyzes limited proteolysis of factor X, converting it to factor Xa. F7a can also convert factor IX to factor IXa, emphasizing its pivotal role in coagulation cascades. Coagulation Factor VII/F7 Protein, Human (HEK293, His) is the recombinant human-derived Coagulation Factor VII/F7 protein, expressed by HEK293 , with C-His labeled tag. The total length of Coagulation Factor VII/F7 Protein, Human (HEK293, His) is 406 a.a., with molecular weight of ~50 kDa.

Background

Coagulation factor VII (F7) plays a crucial role in initiating the extrinsic pathway of blood coagulation. As a serine protease circulating in the blood in its zymogen form, factor VII undergoes activation to factor VIIa through minor proteolysis induced by factor Xa, factor XIIa, factor IXa, or thrombin. In the presence of tissue factor and calcium ions, factor VIIa further catalyzes the limited proteolysis of factor X, converting it to factor Xa. Additionally, in the presence of tissue factor and calcium, factor VIIa is capable of converting factor IX to factor IXa, highlighting its pivotal function in the intricate cascade of coagulation processes.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

HEK293

Tag

C-His

Accession

P08709-1 (A61-P466)

Gene ID
Molecular Construction
N-term
F7 (A61-P466)
Accession # P08709-1
His
C-term
Synonyms
Coagulation factor VII; Eptacog alfa; F7; FVII coagulation protein; SPCA
Molecular Weight

Approximately 50 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

Coagulation Factor VII/F7 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Coagulation Factor VII/F7 Protein, Human (HEK293, His)
Cat. No.:
HY-P74239
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