Contactin-1/CNTN1 Protein, Human (HEK293, His)

Contactin-1/CNTN1 proteins mediate cell surface interactions in the developing nervous system. Contactin-1/CNTN1 Protein, Human (HEK293, His) is the recombinant human-derived Contactin-1/CNTN1, expressed by HEK293, with C-His labeled tag. The total length of Contactin-1/CNTN1 Protein, Human (HEK293, His) is 973 a.a..

Contactin-1 (CNTN1) protein, a key player in nervous system development, facilitates critical cell surface interactions. It plays a significant role in the formation of paranodal axo-glial junctions within myelinated peripheral nerves and contributes to signaling between axons and myelinating glial cells by associating with CNTNAP1. Additionally, CNTN1 participates in the generation of oligodendrocytes, acting as a ligand for NOTCH1. The association with NOTCH1 promotes the activation of NOTCH1, leading to the release of the notch intracellular domain (NICD) and its subsequent translocation to the nucleus. Notably, CNTN1's interaction with TNR induces neuron repulsion and inhibits neurite outgrowth. In its monomeric form, CNTN1 interacts with CNTNAP1 in cis, and it binds to the carbonic-anhydrase like domain of PTPRZ1. Furthermore, CNTN1 forms a complex with NRCAM and PTPRB and interacts with TASOR. These multifaceted interactions underscore the diverse functions of Contactin-1 in orchestrating crucial processes during nervous system development.

Human

HEK293

C-His

Q12860 (E21-S993)

1272  [NCBI]

Partial

EFTWYRRYGHGVSEEDKGFGPIFEEQPINTIYPEESLEGKVSLNCRARASPFPVYKWRMNNGDVDLTSDRYSMVGGNLVINNPDKQKDAGIYYCLASNNYGMVRSTEATLSFGYLDPFPPEERPEVRVKEGKGMVLLCDPPYHFPDDLSYRWLLNEFPVFITMDKRRFVSQTNGNLYIANVEASDKGNYSCFVSSPSITKSVFSKFIPLIPIPERTTKPYPADIVVQFKDVYALMGQNVTLECFALGNPVPDIRWRKVLEPMPSTAEISTSGAVLKIFNIQLEDEGIYECEAENIRGKDKHQARIYVQAFPEWVEHINDTEVDIGSDLYWPCVATGKPIPTIRWLKNGYAYHKGELRLYDVTFENAGMYQCIAENTYGAIYANAELKILALAPTFEMNPMKKKILAAKGGRVIIECKPKAAPKPKFSWSKGTEWLVNSSRILIWEDGSLEINNITRNDGGIYTCFAENNRGKANSTGTLVITDPTRIILAPINADITVGENATMQCAASFDPALDLTFVWSFNGYVIDFNKENIHYQRNFMLDSNGELLIRNAQLKHAGRYTCTAQTIVDNSSASADLVVRGPPGPPGGLRIEDIRATSVALTWSRGSDNHSPISKYTIQTKTILSDDWKDAKTDPPIIEGNMEAARAVDLIPWMEYEFRVVATNTLGRGEPSIPSNRIKTDGAAPNVAPSDVGGGGGRNRELTITWAPLSREYHYGNNFGYIVAFKPFDGEEWKKVTVTNPDTGRYVHKDETMSPSTAFQVKVKAFNNKGDGPYSLVAVINSAQDAPSEAPTEVGVKVLSSSEISVHWEHVLEKIVESYQIRYWAAHDKEEAANRVQVTSQEYSARLENLLPDTQYFIEVGACNSAGCGPPSDMIEAFTKKAPPSQPPRIISSVRSGSRYIITWDHVVALSNESTVTGYKVLYRPDGQHDGKLYSTHKHSIEVPIPRDGEYVVEVRAHSDGGDGVVSQVKIS

Approximately 125 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. /biology-dictionary/d-423mg-cell.html