COX-2 Protein, Human (sf9, His)

COX-2 protein is involved in the alternative pathway of prostaglandin biosynthesis. COX-2 Protein, Human (sf9, His) is the recombinant human-derived COX-2 protein, expressed by Sf9 insect cells , with C-10*His labeled tag.

COX-2 protein serves as a dual functional enzyme in the biosynthesis pathway of prostanoids, critical mediators in the inflammatory response. Acting as both a cyclooxygenase and peroxidase, COX-2 transforms arachidonate (AA) into the hydroperoxy endoperoxide prostaglandin G2 (PGG2) and further reduces it to the hydroxy endoperoxide prostaglandin H2 (PGH2). This catalytic process involves hydrogen abstraction at carbon 13, followed by molecular oxygen insertion to form the characteristic endoperoxide bridge in prostaglandins. COX-2 extends its enzymatic activity to dihomo-gamma-linoleate (DGLA) and eicosapentaenoate (EPA), generating PGH1 and PGH3 as precursors of 1- and 3-series prostaglandins. Additionally, COX-2 contributes to the alternative prostanoid biosynthesis pathway, converting 2-arachidonoyl lysophospholipids and metabolizing 2-arachidonoyl glycerol. The enzyme also plays a role in the generation of resolution phase interaction products, contributing to the synthesis of specialized pro-resolving mediators during inflammatory processes, such as resolvins. This multifaceted functionality highlights the pivotal role of COX-2 in the intricate regulation of prostanoid production and inflammatory signaling.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

C-10*His

P35354/NP_000954.1 (A18-L604)

5743  [NCBI]

Full Length of Mature Protein

ANPCCSHPCQNRGVCMSVGFDQYKCDCTRTGFYGENCSTPEFLTRIKLFLKPTPNTVHYILTHFKGFWNVVNNIPFLRNAIMSYVLTSRSHLIDSPPTYNADYGYKSWEAFSNLSYYTRALPPVPDDCPTPLGVKGKKQLPDSNEIVEKLLLRRKFIPDPQGSNMMFAFFAQHFTHQFFKTDHKRGPAFTNGLGHGVDLNHIYGETLARQRKLRLFKDGKMKYQIIDGEMYPPTVKDTQAEMIYPPQVPEHLRFAVGQEVFGLVPGLMMYATIWLREHNRVCDVLKQEHPEWGDEQLFQTSRLILIGETIKIVIEDYVQHLSGYHFKLKFDPELLFNKQFQYQNRIAAEFNTLYHWHPLLPDTFQIHDQKYNYQQFIYNNSILLEHGITQFVESFTRQIAGRVAGGRNVPPAVQKVSQASIDQSRQMKYQSFNEYRKRFMLKPYESFEELTGEKEMSAELEALYGDIDAVELYPALLVEKPRPDAIFGETMVEVGAPFSLKGLMGNVICSPAYWKPSTFGGEVGFQIINTASIQSLICNNVKGCPFTSFSVPDPELIKTVTINASSSRSGLDDINPTVLLKERSTEL

Approximately 66 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 10% Glycerol, pH 8.0 or 50 mM Tris, 100 mM NaCl, 10% Glycerol, pH 8, 5% Trehalose, 5% Mannitol, 0.01% Tween-80. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.