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CYGB Protein, Rat (His-Myc)

Cat. No.: HY-P72162
COA Handling Instructions

CYGB proteins play a crucial role in cellular defense against oxidative stress and may serve as protective agents that mitigate damage. It involves intracellular processes related to oxygen storage or transfer. CYGB Protein, Rat (His-Myc) is the recombinant rat-derived CYGB protein, expressed by E. coli , with N-10*His, C-Myc labeled tag. The total length of CYGB Protein, Rat (His-Myc) is 190 a.a., with molecular weight of ~31 kDa.

For research use only. We do not sell to patients.

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Description

CYGB proteins play a crucial role in cellular defense against oxidative stress and may serve as protective agents that mitigate damage. It involves intracellular processes related to oxygen storage or transfer. CYGB Protein, Rat (His-Myc) is the recombinant rat-derived CYGB protein, expressed by E. coli , with N-10*His, C-Myc labeled tag. The total length of CYGB Protein, Rat (His-Myc) is 190 a.a., with molecular weight of ~31 kDa.

Background

The CYGB protein appears to fulfill a pivotal role in cellular defense against oxidative stress, potentially acting as a protective agent to mitigate damage. Furthermore, it may play a role in intracellular processes related to oxygen storage or transfer. Notably, CYGB is implicated in the development of liver fibrosis, suggesting its involvement in pathological conditions. Additionally, the protein exhibits peroxidase activity, emphasizing its capacity to participate in redox reactions and contribute to cellular homeostasis.

Species

Rat

Source

E. coli

Tag

N-10*His;C-Myc

Accession

Q921A4 (M1-P190)

Gene ID

170520  [NCBI]

Molecular Construction
N-term
10*His
CYGB (M1-P190)
Accession # Q921A4
C-term
Synonyms
Cygb; StapCytoglobin; Histoglobin; HGb; Stellate cell activation-associated protein
AA Sequence

MEKVPGDMEIERRERNEELSEAERKAVQATWARLYANCEDVGVAILVRFFVNFPSAKQYFSQFKHMEDPLEMERSPQLRKHACRVMGALNTVVENLHDPDKVSSVLALVGKAHALKHKVEPMYFKILSGVILDVIAEEFANDFPVETQKAWTKLRGLIYSHVTAAYKEVGWVQQVPNTTTLPATLPSSGP

Molecular Weight

Approximately 31 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

CYGB Protein, Rat (His-Myc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CYGB Protein, Rat (His-Myc)
Cat. No.:
HY-P72162
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