Fc gamma RIIIA/CD16a Protein, Cynomolgus (Biotinylated, HEK293, His-Avi)

FcγRIIIA/CD16a Protein, a receptor for IgG, optimally activates upon clustered antigen-IgG complex binding, inducing antibody-dependent cellular cytotoxicity (ADCC). It mediates IgG effector functions on natural killer cells, limiting viral load. FcγRIIIA generates memory-like adaptive NK cells, crucial for NK cell activation. As a Fc-binding subunit, it forms signaling complexes with CD247 and/or FCER1G, initiating intracellular pathways. FcγRIIIA costimulates NK cells, triggering lysis independently of IgG binding. It plays a role in antitumor activities, induces TNFA-dependent ADCC, interacts with CD2 for NK cell activation, and interacts with S100A4, inhibiting FCGR3A phosphorylation. Fc gamma RIIIA/CD16a Protein, Cynomolgus (Biotinylated, HEK293, His-Avi) is the recombinant cynomolgus-derived Fc gamma RIIIA/CD16a protein, expressed by HEK293, with C-Avi, C-His labeled tag. The total length of Fc gamma RIIIA/CD16a Protein, Cynomolgus (Biotinylated, HEK293, His-Avi) is 192 a.a., with molecular weight of 36-45 kDa.

The FcγRIIIA/CD16a Protein functions as a receptor for the constant Fc fragment of immunoglobulin gamma (IgG), optimally activated upon binding of clustered antigen-IgG complexes displayed on cell surfaces, leading to antibody-dependent cellular cytotoxicity (ADCC). It mediates IgG effector functions on natural killer (NK) cells, triggering cytokine production, degranulation, and limiting viral load during infection. Additionally, FcγRIIIA is involved in the generation of memory-like adaptive NK cells that efficiently eliminate virus-infected cells via ADCC and regulate NK cell survival and proliferation. As a Fc-binding subunit, it associates with CD247 and/or FCER1G adapters to form functional signaling complexes, initiating intracellular signaling pathways such as phosphatidylinositol 3-kinase signaling and calcium flux, crucial for NK cell activation. FcγRIIIA also costimulates NK cells, triggering lysis of target cells independently of IgG binding. It plays a role in mediating the antitumor activities of therapeutic antibodies and, upon ligation on monocytes, induces tumor necrosis factor-alpha (TNFA)-dependent ADCC of IgG-coated tumor cells. Furthermore, FcγRIIIA interacts with CD2, contributing to NK cell activation and cytotoxicity, and interacts with S100A4, inhibiting PKC-dependent phosphorylation of FCGR3A.

Measured by its binding ability in a functional ELISA. Immobilized Anti-CD16a antibody at 2 μg/mL can bind Biotinylated Cynomolgus CD16. The EC₅₀ is 1.737-10.78 ng/mL.

Cynomolgus

HEK293

C-Avi;C-His

Q8SPW2 (G17-Q208)

102140945  [NCBI]

36-45 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.