Cystathionine gamma-lyase/CTH Protein, Human

CTH protein is a cysteine protein sulfatase that catalyzes the final step of the transsulfuration pathway in a PLP-dependent manner. Cystathionine gamma-lyase/CTH Protein, Human is the recombinant human-derived Cystathionine gamma-lyase/CTH protein, expressed by E. coli , with tag free.

Cystathionine gamma-lyase (CTH) plays a crucial role in the final step of the trans-sulfuration pathway, converting L-methionine to L-cysteine in a pyridoxal-5'-phosphate (PLP)-dependent manner. This process involves cleaving L,L-cystathionine into L-cysteine, ammonia, and 2-oxobutanoate. A portion of the L-cysteine produced through this pathway is utilized for the biosynthesis of the antioxidant glutathione. Beyond its role in L-cystathionine conversion, CTH functions as a cysteine-protein sulfhydrase, mediating sulfhydration of specific cysteine residues in target proteins like GAPDH, PTPN1, and NF-kappa-B subunit RELA, thereby regulating their function. Moreover, CTH utilizes L-cysteine and L-homocysteine to produce the gasotransmitter hydrogen sulfide (H2S), a signaling molecule implicated in various physiological processes such as vasodilation, bone protection, and inflammation. Under conditions of severe hyperhomocysteinemia, CTH can convert L-cysteine and L-homocysteine into lanthionine and homolanthionine, structural homologs of L,L-cystathionine. Additionally, CTH contributes to myogenesis by facilitating H2S biogenesis in skeletal muscle tissue and can accept homoserine as a substrate. Furthermore, it catalyzes the elimination of selenocystathionine to yield selenocysteine, ammonia, and 2-oxobutanoate, a process relevant to dietary selenium metabolism.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

Tag Free

P32929 (M1-S405)

1491  [NCBI]

Full Length

MQEKDASSQGFLPHFQHFATQAIHVGQDPEQWTSRAVVPPISLSTTFKQGAPGQHSGFEYSRSGNPTRNCLEKAVAALDGAKYCLAFASGLAATVTITHLLKAGDQIICMDDVYGGTNRYFRQVASEFGLKISFVDCSKIKLLEAAITPETKLVWIETPTNPTQKVIDIEGCAHIVHKHGDIILVVDNTFMSPYFQRPLALGADISMYSATKYMNGHSDVVMGLVSVNCESLHNRLRFLQNSLGAVPSPIDCYLCNRGLKTLHVRMEKHFKNGMAVAQFLESNPWVEKVIYPGLPSHPQHELVKRQCTGCTGMVTFYIKGTLQHAEIFLKNLKLFTLAESLGGFESLAELPAIMTHASVLKNDRDVLGISDTLIRLSVGLEDEEDLLEDLDQALKAAHPPSGSHS

Approximately 38.0-50.0 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 8% sucrose, 0.05% Tween 80, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.