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Cystatin-8/CST8 Protein, Human (HEK293, Fc)

Cat. No.: HY-P76851
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Cystatin-8/CST8 Protein specializes in sperm development and maturation. Cystatin-8/CST8 Protein, Human (HEK293, Fc) is the recombinant human-derived Cystatin-8/CST8 protein, expressed by HEK293 , with C-hFc labeled tag.

For research use only. We do not sell to patients.

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5 μg $40 In-stock
10 μg $70 In-stock
50 μg $200 In-stock
100 μg $340 In-stock
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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Cystatin-8/CST8 Protein specializes in sperm development and maturation. Cystatin-8/CST8 Protein, Human (HEK293, Fc) is the recombinant human-derived Cystatin-8/CST8 protein, expressed by HEK293 , with C-hFc labeled tag.

Background

Cystatin-8/CST8 Protein plays a specialized role during sperm development and maturation.

Biological Activity

Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC. The IC50 value is 4.188 nM, as measured under the described conditions.

Species

Human

Source

HEK293

Tag

C-hFc

Accession

O60676 (K22-A142)

Gene ID
Molecular Construction
N-term
CST8 (M1-A142)
Accession # O60676
hFc
C-term
Synonyms
Cystatin-related epididymal spermatogenic protein; Cystatin-8; CRES
AA Sequence

KDPKKNETGVLRKLKPVNASNANVKQCLWFAMQEYNKESEDKYVFLVVKTLQAQLQVTNLLEYLIDVEIARSDCRKPLSTNEICAIQENSKLKRKLSCSFLVGALPWNGEFTVMEKKCEDA

Molecular Weight

Approximately 43-55 kDa due to the glycosylation

Purity
  • Greater than 90% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Cystatin-8/CST8 Protein, Human (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Cystatin-8/CST8 Protein, Human (HEK293, Fc)
Cat. No.:
HY-P76851
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