Delta-like protein 4/DLL4 Protein, Mouse (HEK293, Fc)

Delta-like 4/DLL4 protein is a key player in the Notch signaling pathway, acting as a ligand to activate NOTCH1 and NOTCH4. In angiogenesis, DLL4 regulates endothelial cell proliferation, migration, and angiogenic sprouting. Delta-like protein 4/DLL4 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Delta-like protein 4/DLL4 protein, expressed by HEK293 , with C-mFc labeled tag.

The Delta-like protein 4/DLL4 Protein plays a pivotal role in the Notch signaling pathway, serving as a Notch ligand and activating both NOTCH1 and NOTCH4. In the context of angiogenesis, DLL4 assumes a regulatory role by negatively influencing endothelial cell proliferation, migration, and angiogenic sprouting. The protein is essential for retinal progenitor proliferation and is required for suppressing rod fates in late retinal progenitors while contributing to the proper generation of other retinal cell types. Furthermore, during spinal cord neurogenesis, DLL4 inhibits V2a interneuron fate. Its interactions with NOTCH4 and NOTCH1 highlight the molecular basis of its role as a ligand in the Notch signaling cascade, emphasizing its multifaceted functions in orchestrating crucial cellular processes during development.

Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is typically 3.1 μg/mL.

Mouse

HEK293

C-mFc

Q9JI71 (S28-P525)

54485  [NCBI]

Partial

SGIFQLRLQEFVNQRGMLANGQSCEPGCRTFFRICLKHFQATFSEGPCTFGNVSTPVLGTNSFVVRDKNSGSGRNPLQLPFNFTWPGTFSLNIQAWHTPGDDLRPETSPGNSLISQIIIQGSLAVGKIWRTDEQNDTLTRLSYSYRVICSDNYYGESCSRLCKKRDDHFGHYECQPDGSLSCLPGWTGKYCDQPICLSGCHEQNGYCSKPDECICRPGWQGRLCNECIPHNGCRHGTCSIPWQCACDEGWGGLFCDQDLNYCTHHSPCKNGSTCSNSGPKGYTCTCLPGYTGEHCELGLSKCASNPCRNGGSCKDQENSYHCLCPPGYYGQHCEHSTLTCADSPCFNGGSCRERNQGSSYACECPPNFTGSNCEKKVDRCTSNPCANGGQCQNRGPSRTCRCRPGFTGTHCELHISDCARSPCAHGGTCHDLENGPVCTCPAGFSGRRCEVRITHDACASGPCFNGATCYTGLSPNNFVCNCPYGFVGSRCEFPVGLP

80.61 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.