Enolase 1/ENO1 Protein, Human (His)
Based on 1 Customer Validation
The ENO1 protein converts 2-phosphoglycerate to phosphoenolpyruvate and is involved in growth control, hypoxia tolerance, and allergic responses. It acts as a receptor and activator of plasminogen, stimulating immunoglobulin production. ENO1 binds to the myc promoter as a transcriptional repressor and may function as a tumor suppressor. Enolase 1/ENO1 Protein, Human (His) is the recombinant human-derived Enolase 1/ENO1 protein, expressed by E. coli , with C-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The ENO1 protein converts 2-phosphoglycerate to phosphoenolpyruvate and is involved in growth control, hypoxia tolerance, and allergic responses. It acts as a receptor and activator of plasminogen, stimulating immunoglobulin production. ENO1 binds to the myc promoter as a transcriptional repressor and may function as a tumor suppressor. Enolase 1/ENO1 Protein, Human (His) is the recombinant human-derived Enolase 1/ENO1 protein, expressed by E. coli , with C-6*His labeled tag.
Background
The ENO1 Protein operates as a glycolytic enzyme, catalyzing the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to its role in glycolysis, ENO1 is implicated in various processes such as growth control, hypoxia tolerance, and allergic responses. Notably, it displays versatility by potentially functioning in the intravascular and pericellular fibrinolytic system, acting as a receptor and activator of plasminogen on the cell surface of diverse cell types including leukocytes and neurons. ENO1's ability to stimulate immunoglobulin production further underscores its multifaceted functions. Moreover, it binds to the myc promoter and acts as a transcriptional repressor, suggesting a regulatory role in gene expression. Additionally, ENO1 may function as a tumor suppressor, implicating its involvement in cellular processes that extend beyond glycolysis and impact critical aspects of growth control and immune responses.
Verified Bioactivity
Specific activity is 20188 pmol/min/µg, and was obtained by measuring the decrease of NAD that incubate at 37 ºC for 5 minutes in absorbance at 340nm resulting from NADH at pH 6.5.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source E. coli
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Tag C-6*His
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Accession
P06733-1 (M1-K434)
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Molecular Construction
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N-term
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ENO1 (M1-K434)
Accession # P06733-1 -
6*His
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C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
ENO1; Enolase 1, (Alpha); Prev. ENO1L1; NNE; Prev. MPB1; Epididymis Secretory Protein Li 17; Prev. ENO1-IT1; C-Myc Promoter-Binding Protein-1; 2-Phospho-D-Glycerate Hydro-Lyase; MYC Promoter-Binding Protein 1; Phosphopyruvate Hydratase; C-Myc Promoter-Bin
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AA Sequence
MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK
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Predicted Molecular Mass
47.2 kDa
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Molecular Weight
Approximately 49 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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