1. Recombinant Proteins
  2. Fc Receptors
  3. Fc-epsilon Receptor
  4. Fc epsilon RIA
  5. Fc epsilon RIA/FCER1A Protein, Mouse (204a.a, HEK293, Fc)

Fc epsilon RIA/FCER1A Protein, Mouse (204a.a, HEK293, Fc)

Cat. No.: HY-P75212
COA Handling Instructions

Fc epsilon RIA/FCER1A protein is a high-affinity receptor for immunoglobulin epsilon/IgE and is critical for mediating IgE effector function. It activates and differentiates myeloid cells after binding IgE and cross-linking with antigens/allergens, causing mast cells, basophils, and eosinophils to secrete mediators and cytokines. Fc epsilon RIA/FCER1A Protein, Mouse (204a.a, HEK293, Fc) is the recombinant mouse-derived Fc epsilon RIA/FCER1A protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Fc epsilon RIA/FCER1A Protein, Mouse (204a.a, HEK293, Fc) is 181 a.a., with molecular weight of ~64.07 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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  • Documentation

Description

Fc epsilon RIA/FCER1A protein is a high-affinity receptor for immunoglobulin epsilon/IgE and is critical for mediating IgE effector function. It activates and differentiates myeloid cells after binding IgE and cross-linking with antigens/allergens, causing mast cells, basophils, and eosinophils to secrete mediators and cytokines. Fc epsilon RIA/FCER1A Protein, Mouse (204a.a, HEK293, Fc) is the recombinant mouse-derived Fc epsilon RIA/FCER1A protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Fc epsilon RIA/FCER1A Protein, Mouse (204a.a, HEK293, Fc) is 181 a.a., with molecular weight of ~64.07 kDa.

Background

Fc epsilon RIA/FCER1A Protein is a high-affinity receptor for immunoglobulin epsilon/IgE. It plays a crucial role in mediating IgE effector functions in myeloid cells. Upon binding IgE and cross-linking with antigen/allergen, it initiates signaling pathways that activate and differentiate myeloid cells. This activation leads to the secretion of vasoactive amines, lipid mediators, and cytokines by mast cells, basophils, and eosinophils, which contribute to inflammatory responses, tissue remodeling, and cytotoxicity against microbes. The protein is responsible for triggering the immediate hypersensitivity response to allergens, acting as a host defense mechanism against helminth parasites, pathogenic bacteria, and venom toxicity. However, when dysregulated, it can cause harmful, life-threatening allergic and anaphylactic reactions. Structurally, Fc epsilon RIA/FCER1A Protein consists of a tetramer comprising an alpha chain, a beta chain, and two disulfide-linked gamma chains. It interacts with IGHE through its CH3 region.

Biological Activity

Data is not available.

Species

Mouse

Source

HEK293

Tag

C-hFc

Accession

P20489 (A24-Q204)

Gene ID

14125  [NCBI]

Molecular Construction
N-term
FCER1A (A24-Q204)
Accession # P20489
hFc
C-term
Synonyms
Fc epsilon RI alpha; FCE1A; FCER1A; FcERI; FceRIa
AA Sequence

ATEKSVLTLDPPWIRIFTGEKVTLSCYGNNHLQMNSTTKWIHNGTVSEVNSSHLVIVSATVQDSGKYICQKQGLFKSKPVYLNVTQDWLLLQTSADMVLVHGSFDIRCHGWKNWNVRKVIYYRNDHAFNYSYESPVSIREATLNDSGTYHCKGYLRQVKYESDKFRIAVVKAYKCKYYWLQ

Molecular Weight

Approximately 64.07 kDa

Purity
  • Greater than 85% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Fc epsilon RIA/FCER1A Protein, Mouse (204a.a, HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Fc epsilon RIA/FCER1A Protein, Mouse (204a.a, HEK293, Fc)
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HY-P75212
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