1. Recombinant Proteins
  2. CD Antigens Fc Receptors Biotinylated Proteins
  3. Macrophage CD Proteins Monocyte CD Proteins Platelet CD Proteins Dendritic Cell CD Proteins Fc-gamma Receptor
  4. Fc gamma RII/CD32 FCGR2A/CD32a
  5. FCGR2A/CD32a
  6. Fc gamma RIIA/CD32a Protein, Human (Biotinylated, H167R, HEK293, His-Avi)

Fc gamma RIIA/CD32a Protein, Human (Biotinylated, H167R, HEK293, His-Avi)

Cat. No.: HY-P76228
Handling Instructions
Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized human IgG1 at 10 μg/mL (100 μL/well) can bind human CD32A and the EC50 is 1.6 - 3.8 μg/mL. Using the Octet RED System, the affinity constant (Kd) of biotinylated human CD32A (16
Species

Human

Source

HEK293

Tag

C-His;C-Avi

Accession

P12318 (M1-M210)

Gene ID
Synonyms
C-X-C motif chemokine 12; Stromal cell-derived factor 1; CXCL12; SDF-1β
Molecular Weight

Approximately 32 kDa.

Purity

Greater than 97 % as determined by SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Fc gamma RIIA/CD32a Protein, Human (Biotinylated, H167R, HEK293, His-Avi) Related Classifications

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Fc gamma RIIA/CD32a Protein, Human (Biotinylated, H167R, HEK293, His-Avi)
Cat. No.:
HY-P76228
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