1. Recombinant Proteins
  2. CD Antigens Fc Receptors
  3. Macrophage CD Proteins Monocyte CD Proteins Platelet CD Proteins Dendritic Cell CD Proteins Fc-gamma Receptor
  4. Fc gamma RII/CD32 FCGR2A/CD32a
  5. FCGR2A/CD32a
  6. Fc gamma RIIA/CD32a Protein, Human (H167R, HEK293, Fc)

Fc gamma RIIA/CD32a Protein, Human (H167R, HEK293, Fc)

Cat. No.: HY-P75644
SDS COA Handling Instructions

Fc gamma The RIIA/CD32a protein plays a key role as a low-affinity receptor that specifically binds to the Fc region of immunoglobulin gamma. It interacts with IgG to initiate cellular responses against pathogens, which is critical for immune regulation. Fc gamma RIIA/CD32a Protein, Human (H167R, HEK293, Fc) is the recombinant human-derived Fc gamma RIIA/CD32a protein, expressed by HEK293 , with C-hFc labeled tag and H167R, , , , mutation.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
10 μg $209 In-stock
20 μg $334 In-stock
50 μg $635 In-stock
100 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

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Description

Fc gamma The RIIA/CD32a protein plays a key role as a low-affinity receptor that specifically binds to the Fc region of immunoglobulin gamma. It interacts with IgG to initiate cellular responses against pathogens, which is critical for immune regulation. Fc gamma RIIA/CD32a Protein, Human (H167R, HEK293, Fc) is the recombinant human-derived Fc gamma RIIA/CD32a protein, expressed by HEK293 , with C-hFc labeled tag and H167R, , , , mutation.

Background

The Fc gamma RIIA/CD32a protein assumes a pivotal role by specifically binding to the Fc region of immunoglobulins gamma, functioning as a low-affinity receptor. Through its interaction with IgG, Fc gamma RIIA/CD32a initiates cellular responses against pathogens and soluble antigens, illustrating its crucial involvement in immune modulation. Notably, the protein promotes the phagocytosis of opsonized antigens, further contributing to immune defense mechanisms. Additionally, Fc gamma RIIA/CD32a engages in interactions with IGHG1, INPP5D/SHIP1, INPPL1/SHIP2, APCS, FGR, and HCK, indicating its participation in intricate signaling pathways and the regulation of its cellular functions. These multifaceted interactions underscore the significance of Fc gamma RIIA/CD32a in orchestrating diverse immune responses.

Biological Activity

Measured by its binding abilty in a functional ELISA. Immobilized human CD32a-Fc at 10 μg/mL(100 μl/well) can bind biotinylated human lgG1 with a linear range of 0.625-10 μg/mL.

Species

Human

Source

HEK293

Tag

C-hFc

Accession

P12318-1 (Q34-I218, H167R)

Gene ID
Molecular Construction
N-term
CD32a (Q34-I218, H167R)
Accession # P12318-1
hFc
C-term
Synonyms
Low Affinity Immunoglobulin Gamma Fc Region Receptor II-a; CD32; FCGR2A; FCG2; IGFR2
Molecular Weight

Approximately 55 kDa

Purity

Greater than 90% as determined by Tris-Bis PAGE.

Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

Fc gamma RIIA/CD32a Protein, Human (H167R, HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Fc gamma RIIA/CD32a Protein, Human (H167R, HEK293, Fc)
Cat. No.:
HY-P75644
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