1. Recombinant Proteins
  2. CD Antigens Fc Receptors Biotinylated Proteins
  3. T Cell CD Proteins NK Cell CD Proteins Macrophage CD Proteins Monocyte CD Proteins Stem Cell CD Proteins Dendritic Cell CD Proteins Fc-gamma Receptor
  4. FcγRIIIB/CD16b Fc gamma RIII/CD16
  5. Fc gamma RIIIB/CD16b Protein, Human (Biotinylated, NA2, HEK293, His-Avi)

Fc gamma RIIIB/CD16b Protein, Human (Biotinylated, NA2, HEK293, His-Avi)

Cat. No.: HY-P78122
Handling Instructions

For research use only. We do not sell to patients.

Protein Expression Service

Size Price Stock Quantity
20 μg $245 In-stock
Estimated Time of Arrival: December 31
50 μg $540 In-stock
Estimated Time of Arrival: December 31
100 μg $918 Get quote
250 μg   Get quote  

* Please select Quantity before adding items.

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity
Biotinylated Human Fc gamma RIIIB (NA2) His captured on CM5 Chip via Anti-His Antibody can bind Rituximab hFc with an affinity constant of 4.3μM as determined in SPR assay.
Species

Human

Source

HEK293

Tag

C-Avi;C-His

Accession

O75015 (G17-S200)

Gene ID
Synonyms
CD16; Fc gamma RIIIB; FCG3; Fc-gamma receptor IIIb (CD 16); Fc-gamma RIIIb; Fc-gamma RIII-beta; FCGR3B; FcgRIIIB; FCRIIIB; IGFR3; IgG Fc receptor III-1; CD16b (NA2); CD16B; FCG3B; FCG3; FCGR3; FCR-10; Fc-gamma RIII-β
Molecular Weight

47-53 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE. Greater than 95% as determined by HPLC.

Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Fc gamma RIIIB/CD16b Protein, Human (Biotinylated, NA2, HEK293, His-Avi)
Cat. No.:
HY-P78122
Quantity:
MCE Japan Authorized Agent: