FCRN-B2M Heterodimer Protein, Human (Biotinylated, HEK293, His-Avi)

FCGRT is an IgG Fc receptor. Fc receptor expression is up-regulated by pro-inflammatory cytokines TNF and down-regulated by IFN-γ. FCGRT plays a role in regulating IgG and serum albumin conversion. Overexpression of FCGRT is also associated with poor prognosis of tumors. FCRN-B2M Heterodimer Protein, Human (Biotinylated, HEK293, His-Avi) is a recombinant protein dimer complex containing human-derived FCRN-B2M protein, expressed by HEK293 , with C-Avi, C-6*His labeled tag.

IgG receptor FcRn large subunit p51 is an IgG Fc receptor with a molecular structure similar to MHC Class I and also binds to β-2 microglobulin. In rodents, FcRn was originally thought to be a receptor that trantranks maternal immunoglobulin G (IgG) from mother to newborn offspring via breast milk and is therefore known as a neonatal Fc receptor. FcRn has also been shown to play a role in regulating IgG and serum albumin conversion, and neonatal Fc receptor expression is up-regulated by pro-inflammatory cytokines TNF and down-regulated by IFN-γ. In the acidic endosomes of endothelial and hematopoietic cells, monomer IgG binds to FcRn, circulates IgG to the cell surface, where it is released into the circulation, regulating, in addition to IgG, homeostasis of the circulating protein albumin /ALB. The up-regulated expression of FCGRT in bladder cancer may serve as a key neutrophil gene associated with poor prognosis. FCGRT overexpression was also associated with decreased PD-L1 expression and decreased tumor mutation load (TMB) level^{[1][2][3][4][5][6]}.

1. Loaded Biotinylated Human FcRn Heterodimer-His-Avi on HIS1K Biosensor, can bind Anti-Human HER2 mAb (Human IgG1) with an affinity constant of 0.11 μM as determined in BLI assay.
2. Loaded Rozanolixizumab (HY-P9979) on AHC2 biosensor, can bind FCRN-B2M Protein, Human (Biotinylated, HEK293, His-Avi) with an affinity constant of 1.430E-10 M as determined in BLI assay.

• 
  Loaded Rozanolixizumab (HY-P9979) on AHC2 biosensor, can bind FCRN-B2M Protein, Human (Biotinylated, HEK293, His-Avi) with an affinity constant of 1.430E-10 M as determined in BLI assay.

Human

HEK293

C-Avi;C-6*His

AAF72596 (A24-S297)&P61769 (I21-M119)

2217  [NCBI]&567  [NCBI]

Biotin

The single lysine residue in Avitag is biotinylated through an enzymatic reaction.

AESHLSLLYHLTAVSSPAPGTPAFWVSGWLGPQQYLSYNSLRGEAEPCGAWVWENQVSWYWEKETTDLRIKEKLFLEAFKALGGKGPYTLQGLLGCELGPDNTSVPTAKFALNGEEFMNFDLKQGTWGGDWPEALAISQRWQQQDKAANKELTFLLFSCPHRLREHLERGRGNLEWKEPPSMRLKARPSSPGFSVLTCSAFSFYPPELQLRFLRNGLAAGTGQGDFGPNSDGSFHASSSLTVKSGDEHHYCCIVQHAGLAQPLRVELESPAKSS&IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM

35&13 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 6% Sucrose, 4% Mannitol, 0.05% Tween 80, pH 7.8.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. /biology-dictionary/sk-n-dz-cell.html

  [2]. imister NE, et al. Cloning and expression of the neonatal rat intestinal Fc receptor, a major histocompatibility complex class I antigen homolog. Cold Spring Harb Symp Quant Biol. 1989;54 Pt 1:571-80.  [Content Brief]

  [3]. Ghetie V, et al. Abnormally short serum half-lives of IgG in beta 2-microglobulin-deficient mice. Eur J Immunol. 1996 Mar;26(3):690-6.  [Content Brief]

  [4]. Yang R, et al. Neutrophil-related genes predict prognosis and response to immune checkpoint inhibitors in bladder cancer. Front Pharmacol. 2022 Oct 21;13:1013672.  [Content Brief]

  [5]. West AP Jr, et al. Crystal structure and immunoglobulin G binding properties of the human major histocompatibility complex-related Fc receptor(,). Biochemistry. 2000 Aug 15;39(32):9698-708.  [Content Brief]

  [6]. Pyzik M, et al FcRn regulates albumin homeostasis and susceptibility to liver injury. Proc Natl Acad Sci U S A. 2017 Apr 4;114(14):E2862-E2871.  [Content Brief]

  [7]. Story CM, et al. A major histocompatibility complex class I-like Fc receptor cloned from human placenta: possible role in transfer of immunoglobulin G from mother to fetus. J Exp Med. 1994 Dec 1;180(6):2377-81.  [Content Brief]