GAD2/GAD65 Protein, Mouse (sf9, His-GST)

GAD2/GAD65 Protein is a key enzyme in neurotransmitter metabolism, specifically catalyzing the production of gamma-aminobutyric acid (GABA). Meanwhile, GAD2 also has glutamate binding activity, glutamate decarboxylase activity, and pyridoxal phosphate binding activity. GAD2 is expressed in several structures, including the genitourinary system, the gastrointestinal nervous system, and sensory organs. It can be used to study epilepsy and type 1 diabetes. GAD2/GAD65 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived GAD2/GAD65 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

Glutamate decarboxylase 2 (GAD2) is a key enzyme in neurotransmitter metabolism, specifically catalyzing the production of gamma-aminobutyric acid (GABA). GABA is a crucial inhibitory neurotransmitter in the central nervous system, playing a pivotal role in regulating neuronal excitability and maintaining the balance between excitation and inhibition in the brain. As the catalyst for GABA synthesis, GAD2 plays a fundamental role in modulating neurotransmission and influencing various physiological processes, highlighting its importance in the intricate network of neural signaling within the nervous system. Meanwhile, GAD2 also has glutamate binding activity, glutamate decarboxylase activity, and pyridoxal phosphate binding activity. GAD2 is expressed in several structures, including the genitourinary system, the gastrointestinal nervous system, and sensory organs. In addition, GAD2 can be used to study epilepsy and type 1 diabetes^[1].

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

Sf9 insect cells

N-His;N-GST

Q548L4 (M1-L585)

14417  [NCBI]

Full Length

MASPGSGFWSFGSEDGSADPENPGTARAWCQVAQKFTGGIGNKLCALLYGDSGKPAEGGGSVTSRAATGKVACTCDQKPCNCPKGDVNYAFLHATDLLPACDGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWELADQPQNLEEILTHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWLTSTANTNMFTYEIAPVFVLLEYVTLKKMREIIGWPGGSGDGIFSPGGAISNMYAMLIARYKMFPEVKEKGMAAVPRLIAFTSEHSHFSLKKGAAALGIGTDSVILIKCDERGKMIPSDLERRILEVKQKGFVPFLVSATAGTTVYGAFDPLLAVADICKKYKIWMHVDAAWGGGLLMSRKHKWKLSGVERANSVTWNPHKMMGVPLQCSALLVREEGLMQSCNQMHASYLFQQDKHYDLSYDTGDKALQCGRHVDVFKLWLMWRAKGTTGFEAHIDKCLELAEYLYTIIKNREGYEMVFDGKPQHTNVCFWFVPPSLRTLEDNEERMSRLSKVAPVIKARMMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL

Approximately 80 kDa,based on SDS-PAGE under reducing conditions.

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 0.5 mM GSH, 3 mM DTT, 10% glycerol, pH 8.0, 5% trehalose, 5% mannitol, 0.01% Tween 80.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.