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Galectin-1/LGALS1 Protein, Mouse (HEK293, Fc)

Cat. No.: HY-P78301
COA Handling Instructions

Galectin-1/LGALS1 protein is a β-galactoside-binding lectin that regulates apoptosis, cell proliferation, and differentiation by inhibiting CD45 protein phosphatase.It prevents Lyn kinase dephosphorylation, induces T cell apoptosis as a homodimer, and interacts with CD2, CD3, CD4, CD6, CD7, CD43, ALCAM and CD45.Galectin-1/LGALS1 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Galectin-1/LGALS1 protein, expressed by HEK293 , with N-hFc labeled tag.

For research use only. We do not sell to patients.

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Description

Galectin-1/LGALS1 protein is a β-galactoside-binding lectin that regulates apoptosis, cell proliferation, and differentiation by inhibiting CD45 protein phosphatase.It prevents Lyn kinase dephosphorylation, induces T cell apoptosis as a homodimer, and interacts with CD2, CD3, CD4, CD6, CD7, CD43, ALCAM and CD45.Galectin-1/LGALS1 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Galectin-1/LGALS1 protein, expressed by HEK293 , with N-hFc labeled tag.

Background

Galectin-1/LGALS1 Protein functions as a versatile lectin with a high affinity for beta-galactoside and a diverse range of complex carbohydrates. Its pivotal role extends to the regulation of apoptosis, cell proliferation, and cell differentiation. Notably, Galectin-1/LGALS1 acts as an inhibitor of CD45 protein phosphatase activity, thereby preventing the dephosphorylation of Lyn kinase. Furthermore, it serves as a potent inducer of T-cell apoptosis and exists as a homodimer. The protein engages in various interactions, binding to LGALS3BP and interacting with cell surface proteins such as CD2, CD3, CD4, CD6, CD7, CD43, ALCAM, and CD45. Galectin-1/LGALS1 also forms associations with laminin through poly-N-acetyllactosamine binding and interacts with SUSD2. A noteworthy interaction occurs with the cargo receptor TMED10, facilitating translocation from the cytoplasm into the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) and subsequent secretion.

Species

Mouse

Source

HEK293

Tag

N-hFc

Accession

P16045 (A2-E135)

Gene ID
Molecular Construction
N-term
hFc
LGALS1 (A2-E135)
Accession # P16045
C-term
Synonyms
Galectin-1; HLBP14; S-Lac lectin 1; LGALS1; GAL1; GBP; DKFZp686E23103
Molecular Weight

45-52 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Galectin-1/LGALS1 Protein, Mouse (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Galectin-1/LGALS1 Protein, Mouse (HEK293, Fc)
Cat. No.:
HY-P78301
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