GAS6 Protein, Human (HEK293, Fc)

The GAS6 protein is a ligand for AXL, TYRO3 and MER receptors and affects a variety of cellular processes. GAS6/AXL signaling promotes endothelial cell survival, aids cytokine signaling in natural killer cell development, supports liver regeneration, affects gonadotropin-releasing hormone neuron survival and migration, modulates platelet activation, and regulates thrombotic responses. GAS6 Protein, Human (HEK293, Fc) is the recombinant human-derived GAS6 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of GAS6 Protein, Human (HEK293, Fc) is 648 a.a., with molecular weight of 110-120 kDa.

GAS6, a ligand for tyrosine-protein kinase receptors AXL, TYRO3, and MER, is implicated in diverse cellular processes including cell growth, survival, adhesion, and migration. GAS6/AXL signaling contributes to endothelial cell survival under acidic conditions by preventing apoptosis, facilitates optimal cytokine signaling during human natural killer cell development, plays a role in hepatic regeneration, influences gonadotropin-releasing hormone neuron survival and migration, regulates platelet activation, and modulates thrombotic responses. Additionally, in microbial infections, GAS6 can act as a bridge between virus envelope phosphatidylserine and the TAM receptor tyrosine kinase Axl, facilitating viral entry through apoptotic mimicry. This unique function extends to its involvement in the entry processes of diverse viruses, including Dengue, Vaccinia, ebolavirus, and marburgvirus, highlighting the multifaceted roles of GAS6 in cellular and infectious contexts.

Immobilized Human AXL-His at 5 μg/mL (100 μl/well) can bind Human GAS6-Fc. The ED₅₀ of Human GAS6-Fc is ≤27 μg/mL.

Human

HEK293

C-hFc

Q14393 (A31-A678)

2621  [NCBI]

ALLPAREATQFLRPRQRRAFQVFEEAKQGHLERECVEELCSREEAREVFENDPETDYFYPRYLDCINKYGSPYTKNSGFATCVQNLPDQCTPNPCDRKGTQACQDLMGNFFCLCKAGWGGRLCDKDVNECSQENGGCLQICHNKPGSFHCSCHSGFELSSDGRTCQDIDECADSEACGEARCKNLPGSYSCLCDEGFAYSSQEKACRDVDECLQGRCEQVCVNSPGSYTCHCDGRGGLKLSQDMDTCEDILPCVPFSVAKSVKSLYLGRMFSGTPVIRLRFKRLQPTRLVAEFDFRTFDPEGILLFAGGHQDSTWIVLALRAGRLELQLRYNGVGRVTSSGPVINHGMWQTISVEELARNLVIKVNRDAVMKIAVAGDLFQPERGLYHLNLTVGGIPFHEKDLVQPINPRLDGCMRSWNWLNGEDTTIQETVKVNTRMQCFSVTERGSFYPGSGFAFYSLDYMRTPLDVGTESTWEVEVVAHIRPAADTGVLFALWAPDLRAVPLSVALVDYHSTKKLKKQLVVLAVEHTALALMEIKVCDGQEHVVTVSLRDGEATLEVDGTRGQSEVSAAQLQERLAVLERHLRSPVLTFAGGLPDVPVTSAPVTAFYRGCMTLEVNRRLLDLDEAAYKHSDITAHSCPPVEPAAA

110-120 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.