GAS6 Protein, Human (HEK293, Fc)

GAS6 Protein is a secreted protein located outside the cell membrane. GAS6 Protein is a ligand for tyrosine protein kinase receptors AXL, TYRO3, and MER, and its signal transduction is related to cell growth and survival, cell adhesion, and cell migration. GAS6 is associated with tumor cell growth, metastasis, invasion, epithelial mesenchymal transition, angiogenesis, drug resistance, immune regulation, and stem cell maintenance after binding to AXL. GAS6 Protein, Human (HEK293, Fc) is a recombinant GAS6 protein with an Fc tag, expressed by HEK293^[1][2].

GAS6 Protein is encoded by the growth arrest-specific gene 6 (Gas6) and belongs to the vitamin K-dependent protein family^[2].
GAS6 Protein-AXL can regulate various signaling pathways like PI3K/AKT/mTOR, JAK/STAT, NF-κB, and RAS/RAF/MEK/ERK. It plays a role in tumor cell survival, anti-apoptotic signaling, mitosis, migration, invasion, drug resistance, angiogenesis, and the tumor-host relationship^[2].
GAS6 Protein-AXL can promote the survival of endothelial cells, regulate the optimal cytokine signaling during the development of human natural killer cells, support liver regeneration, assist the survival and migration of gonadotropin-releasing hormone neurons, and facilitate platelet activation or thrombotic responses; it can also induce actin remodeling, driving membrane folding, giant cell phagocytosis, and cancer cell invasion^[1][3].
GAS6 Protein signaling plays a beneficial role in septic heart conditions by inhibiting inflammation, oxidative stress, mitochondrial damage, and ER stress, with NLRP3 being the main molecule mediating GAS6 signaling^[4].
GAS6 Protein mediates the binding of viruses to target cells by bridging the phosphatidylserine on the viral particle to the TAM receptor tyrosine kinase AxI on the target cell, facilitating viral entry^[5].
GAS6 Protein is closely related to pathological processes like nephritis, tumors, viral infections, and cardiovascular diseases^[1][4][5].

GAS6 Protein can trigger membrane folding in human glioblastoma LN229 cells, manifested as the formation of peripheral folds (PR) and circular dorsal folds (CDR)^[1].
GAS6 Protein (25-100 ng/mL, 3 h) can inhibit the expression of inflammatory factors and NLRP3, ASC proteins, thereby reducing LPS induced inflammation in HL-1 cells^[4].

Loss of GAS6 Protein expression in GAS6 knockout (GAS6 KO) mice leads to exacerbation of septic myocardial injury in septic mice^[4].
GAS6 Protein (2 μg; once daily; 10 days; i.p.) induces erythropoiesis and alleviates anemia in GAS6^-/-anemic mice^[6].
GAS6 Protein (2 μg; once daily; 4 days; injection) can increase proteinuria and the number of PCNA positive cells in each glomerulus of GAS6^-/-nephrotoxic nephritis mice, thereby promoting the development of nephritis^[7].

Immobilized Human AXL-His at 5 μg/mL (100 μl/well) can bind Human GAS6. The ED₅₀ for this effect is ≤27 μg/mL.

• 
  Immobilized Human AXL (HY-P74407) at 5 μg/mL (100 μL/well) on the plate can bind Biotinylated Human GAS6. The ED₅₀ for this effect is 2.042 μg/mL.

Human

HEK293

C-hFc

Q14393-2 (A31-A678)

2621  [NCBI]

Full Length of Isoform-2 Mature Protein

ALLPAREATQFLRPRQRRAFQVFEEAKQGHLERECVEELCSREEAREVFENDPETDYFYPRYLDCINKYGSPYTKNSGFATCVQNLPDQCTPNPCDRKGTQACQDLMGNFFCLCKAGWGGRLCDKDVNECSQENGGCLQICHNKPGSFHCSCHSGFELSSDGRTCQDIDECADSEACGEARCKNLPGSYSCLCDEGFAYSSQEKACRDVDECLQGRCEQVCVNSPGSYTCHCDGRGGLKLSQDMDTCEDILPCVPFSVAKSVKSLYLGRMFSGTPVIRLRFKRLQPTRLVAEFDFRTFDPEGILLFAGGHQDSTWIVLALRAGRLELQLRYNGVGRVTSSGPVINHGMWQTISVEELARNLVIKVNRDAVMKIAVAGDLFQPERGLYHLNLTVGGIPFHEKDLVQPINPRLDGCMRSWNWLNGEDTTIQETVKVNTRMQCFSVTERGSFYPGSGFAFYSLDYMRTPLDVGTESTWEVEVVAHIRPAADTGVLFALWAPDLRAVPLSVALVDYHSTKKLKKQLVVLAVEHTALALMEIKVCDGQEHVVTVSLRDGEATLEVDGTRGQSEVSAAQLQERLAVLERHLRSPVLTFAGGLPDVPVTSAPVTAFYRGCMTLEVNRRLLDLDEAAYKHSDITAHSCPPVEPAAA

Approximately 100-120 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Zdżalik-Bielecka D, et al. The GAS6-AXL signaling pathway triggers actin remodeling that drives membrane ruffling, macropinocytosis, and cancer-cell invasion. Proc Natl Acad Sci U S A. 2021 Jul 13;118(28):e2024596118.  [Content Brief]

  [2]. Zhu C, et al. AXL receptor tyrosine kinase as a promising anti-cancer approach: functions, molecular mechanisms and clinical applications. Mol Cancer. 2019 Nov 4;18(1):153.  [Content Brief]

  [3]. Park IK, et al. The Axl/Gas6 pathway is required for optimal cytokine signaling during human natural killer cell development. Blood. 2009 Mar 12;113(11):2470-7.  [Content Brief]

  [4]. Ji T, et al. GAS6 attenuates sepsis-induced cardiac dysfunction through NLRP3 inflammasome-dependent mechanism. Free Radic Biol Med. 2024 Jan;210:195-211.  [Content Brief]

  [5]. Morizono K, et al. The soluble serum protein Gas6 bridges virion envelope phosphatidylserine to the TAM receptor tyrosine kinase Axl to mediate viral entry. Cell Host Microbe. 2011 Apr 21;9(4):286-98.  [Content Brief]

  [6]. Angelillo-Scherrer A, et al. Role of Gas6 in erythropoiesis and anemia in mice. J Clin Invest. 2008 Feb;118(2):583-96.  [Content Brief]

  [7]. Yanagita M, et al. Essential role of Gas6 for glomerular injury in nephrotoxic nephritis. J Clin Invest. 2002 Jul;110(2):239-46.  [Content Brief]