Gastric lipase Protein, Human (HEK293, His, solution)

Gastric lipase is a key enzyme in the digestive process and plays a key role in the hydrolysis of triacylglycerols into free fatty acids, diacylglycerols, monoacylglycerols, and glycerol. This enzyme activity contributes significantly to the breakdown of dietary fat in the gastric environment. Gastric lipase Protein, Human (HEK293, His) is the recombinant human-derived Gastric lipase protein, expressed by HEK293 , with C-6*His labeled tag.

Gastric lipase is an enzyme that catalyzes the hydrolysis of triacylglycerols, breaking them down into free fatty acids, diacylglycerol, monoacylglycerol, and glycerol. This hydrolytic activity plays a crucial role in the digestion of dietary fats in the stomach. Gastric lipase exhibits a preference for hydrolyzing the sn-3 position of triacylglycerol molecules. By selectively targeting specific bonds within triacylglycerols, gastric lipase contributes to the generation of smaller lipid molecules that can be further processed and absorbed during the digestive process. It has to emphasize the enzyme's substrate specificity and its significance in the initial steps of fat digestion.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

AAI12273.1 (L20-K398)

8513  [NCBI]

Partial

LFGKLHPGSPEVTMNISQMITYWGYPNEEYEVVTEDGYILEVNRIPYGKKNSGNTGQRPVVFLQHGLLASATNWISNLPNNSLAFILADAGYDVWLGNSRGNTWARRNLYYSPDSVEFWAFSFDEMAKYDLPATIDFIVKKTGQKQLHYVGHSQGTTIGFIAFSTNPSLAKRIKTFYALAPVATVKYTKSLINKLRFVPQSLFKFIFGDKIFYPHNFFDQFLATEVCSREMLNLLCSNALFIICGFDSKNFNTSRLDVYLSHNPAGTSVQNMFHWTQAVKSGKFQAYDWGSPVQNRMHYDQSQPPYYNVTAMNVPIAVWNGGKDLLADPQDVGLLLPKLPNLIYHKEIPFYNHLDFIWAMDAPQEVYNDIVSMISEDKK

Approximately 50 kDa,based on SDS-PAGE under reducing conditions.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 25 mM Tris-HCl, 100 mM Glycine, 10% glycerol, pH 7.3.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.