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  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. GGH/Glutamyl hydrolase gamma Protein, Human (HEK293, His)

GGH/Glutamyl hydrolase gamma Protein, Human (HEK293, His)

Cat. No.: HY-P76953
COA Handling Instructions

GGH (Glutamyl hydrolase gamma) protein hydrolyzes polyglutamate sidechains in pteroylpolyglutamates, progressively removing gamma-glutamyl residues to produce pteroyl-alpha-glutamate (folic acid) and free glutamate. Studies indicate GGH's crucial role in modulating the bioavailability of dietary pteroylpolyglutamates and its significance in the metabolism of both pteroylpolyglutamates and antifolates. GGH/Glutamyl hydrolase gamma Protein, Human (HEK293, His) is the recombinant human-derived GGH/Glutamyl hydrolase gamma protein, expressed by HEK293 , with C-His, C-6*His labeled tag. The total length of GGH/Glutamyl hydrolase gamma Protein, Human (HEK293, His) is 294 a.a., with molecular weight of ~40 KDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

GGH (Glutamyl hydrolase gamma) protein hydrolyzes polyglutamate sidechains in pteroylpolyglutamates, progressively removing gamma-glutamyl residues to produce pteroyl-alpha-glutamate (folic acid) and free glutamate. Studies indicate GGH's crucial role in modulating the bioavailability of dietary pteroylpolyglutamates and its significance in the metabolism of both pteroylpolyglutamates and antifolates. GGH/Glutamyl hydrolase gamma Protein, Human (HEK293, His) is the recombinant human-derived GGH/Glutamyl hydrolase gamma protein, expressed by HEK293 , with C-His, C-6*His labeled tag. The total length of GGH/Glutamyl hydrolase gamma Protein, Human (HEK293, His) is 294 a.a., with molecular weight of ~40 KDa.

Background

GGH (Glutamyl hydrolase gamma) protein is instrumental in the hydrolysis of polyglutamate sidechains present in pteroylpolyglutamates. Its enzymatic activity involves the progressive removal of gamma-glutamyl residues from pteroylpoly-gamma-glutamate, resulting in the generation of pteroyl-alpha-glutamate (folic acid) and free glutamate. This process, as elucidated by studies, suggests that GGH may hold a pivotal role in modulating the bioavailability of dietary pteroylpolyglutamates, and also plays a significant role in the metabolism of both pteroylpolyglutamates and antifolates.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

HEK293

Tag

C-His;C-6*His

Accession

Q92820/NP_003869.1(M25-D318)

Gene ID
Molecular Construction
N-term
GGH (M25-D318)
Accession # Q92820/NP_003869.1
His
C-term
Synonyms
Gamma-glutamyl hydrolase; Conjugase; GH; Gamma-Glu-X carboxypeptidase
AA Sequence

RPHGDTAKKPIIGILMQKCRNKVMKNYGRYYIAASYVKYLESAGARVVPVRLDLTEKDYEILFKSINGILFPGGSVDLRRSDYAKVAKIFYNLSIQSFDDGDYFPVWGTCLGFEELSLLISGECLLTATDTVDVAMPLNFTGGQLHSRMFQNFPTELLLSLAVEPLTANFHKWSLSVKNFTMNEKLKKFFNVLTTNTDGKIEFISTMEGYKYPVYGVQWHPEKAPYEWKNLDGISHAPNAVKTAFYLAEFFVNEARKNNHHFKSESEEEKALIYQFSPIYTGNISSFQQCYIFD

Molecular Weight

Approximately 40 kDa.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

GGH/Glutamyl hydrolase gamma Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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GGH/Glutamyl hydrolase gamma Protein, Human (HEK293, His)
Cat. No.:
HY-P76953
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