GPNMB/Osteoactivin Protein, Human (Isoform 2, HEK293, Fc)

GPNMB, also known as osteoactivin, is a protein thought to have a potential role as a melanin-producing enzyme. Melanogenic enzymes are involved in the production of melanin, the pigment that determines skin, hair and eye color. GPNMB/Osteoactivin Protein, Human (Isoform 2, HEK293, Fc) is the recombinant human-derived GPNMB/Osteoactivin protein, expressed by HEK293 , with C-hFc labeled tag.

GPNMB, also referred to as Osteoactivin, is a protein that has been proposed to have a potential role as a melanogenic enzyme. Melanogenic enzymes are involved in the production of melanin, the pigment responsible for skin, hair, and eye color. While the exact mechanism of action of GPNMB in melanin synthesis is not fully understood, studies have suggested its involvement in this process. Further research is needed to elucidate the specific functions and regulatory mechanisms of GPNMB in melanogenesis and to determine its significance in melanin production.

Measured  by its binding ability in a functional ELISA. Immobilized Recombinant Human  Integrin alpha V beta 3 at 2 μg/mL (100 μL/well) can bind Biotinylated Human  GPNMB with an ED₅₀of 1.557 µg/mL.

Human

HEK293

C-hFc

Q14956-2 (A22-P474)

10457  [NCBI]

Partial

AKRFHDVLGNERPSAYMREHNQLNGWSSDENDWNEKLYPVWKRGDMRWKNSWKGGRVQAVLTSDSPALVGSNITFAVNLIFPRCQKEDANGNIVYEKNCRNEAGLSADPYVYNWTAWSEDSDGENGTGQSHHNVFPDGKPFPHHPGWRRWNFIYVFHTLGQYFQKLGRCSVRVSVNTANVTLGPQLMEVTVYRRHGRAYVPIAQVKDVYVVTDQIPVFVTMFQKNDRNSSDETFLKDLPIMFDVLIHDPSHFLNYSTINYKWSFGDNTGLFVSTNHTVNHTYVLNGTFSLNLTVKAAAPGPCPPPPPPPRPSKPTPSLGPAGDNPLELSRIPDENCQINRYGHFQATITIVEGILEVNIIQMTDVLMPVPWPESSLIDFVVTCQGSIPTEVCTIISDPTCEITQNTVCSPVDVDEMCLLTVRRTFNGSGTYCVNLTLGDDTSLALTSTLISVP

Approximately 107.5 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% tween 80.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.