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  5. GUCY2C/Guanylyl cyclase C Protein, Mouse (HEK293, N-His, C-Myc)

GUCY2C/Guanylyl cyclase C Protein, Mouse (HEK293, N-His, C-Myc)

Cat. No.: HY-P700603
Handling Instructions

GUCY2C, a vital guanylyl cyclase, catalyzes the synthesis of cyclic cGMP from GTP. GUCY2C/Guanylyl cyclase C Protein, Mouse (HEK293, N-His, C-Myc) is the recombinant mouse-derived GUCY2C/Guanylyl cyclase C protein, expressed by HEK293 , with C-Myc, N-10*His labeled tag. The total length of GUCY2C/Guanylyl cyclase C Protein, Mouse (HEK293, N-His, C-Myc) is 414 a.a., with molecular weight of 52.1 kDa.

For research use only. We do not sell to patients.

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Description

GUCY2C, a vital guanylyl cyclase, catalyzes the synthesis of cyclic cGMP from GTP. GUCY2C/Guanylyl cyclase C Protein, Mouse (HEK293, N-His, C-Myc) is the recombinant mouse-derived GUCY2C/Guanylyl cyclase C protein, expressed by HEK293 , with C-Myc, N-10*His labeled tag. The total length of GUCY2C/Guanylyl cyclase C Protein, Mouse (HEK293, N-His, C-Myc) is 414 a.a., with molecular weight of 52.1 kDa.
Background

GUCY2C, a guanylyl cyclase, plays a crucial role in catalyzing the synthesis of cyclic (cGMP) from GTP.
Species

Mouse
Source

HEK293
Tag

C-Myc;N-10*His
Accession

Q3UWA6 (V20-M433)
Gene ID

14917  [NCBI]
Molecular Construction
N-term
10*His
GUCY2C (V20-M433)
Accession # Q3UWA6
C-term
Synonyms
Guanylyl cyclase C; GC-C; STAR; GUCY2C; GUC2C; STA receptor; DIAR6; EC 4.6.1; GCC; GUC2CEC 4.6.1.2; MUCIL
AA Sequence

VFWASQVRQNCRNGSYEISVLMMDNSAYKEPMQNLREAVEEGLDIVRKRLREADLNVTVNATFIYSDGLIHKSGDCRSSTCEGLDLLREITRDHKMGCALMGPSCTYSTFQMYLDTELNYPMISAGSYGLSCDYKETLTRILPPARKLMYFLVDFWKVNNASFKPFSWNSSYVYKNGSEPEDCFWYLNALEAGVSYFSEVLNFKDVLRRSEQFQEILTGHNRKSNVIVMCGTPESFYDVKGDLQVAEDTVVILVDLFSNHYFEENTTAPEYMDNVLVLTLPSEQSTSNTSVAERFSSGRSDFSLAYLEGTLLFGHMLQTFLENGENVTGPKFARAFRNLTFQGFAGPVTLDDSGDIDNIMSLLYVSLDTRKYKVLMKYDTHKNKTIPVAENPNFIWKNHKLPNDVPGLGPQILM

Molecular Weight

52.1 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

GUCY2C/Guanylyl cyclase C Protein, Mouse (HEK293, N-His, C-Myc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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