HARS/HisRS Protein, Human (sf9, His)

HARS, a pivotal enzyme, orchestrates the ATP-dependent ligation of histidine to the 3'-end of its corresponding tRNA by forming an aminoacyl-adenylate intermediate (His-AMP). This critical step in protein synthesis ensures the accurate incorporation of histidine into nascent polypeptide chains. Beyond its fundamental role in translation, HARS also emerges as a multifunctional player, contributing to axon guidance processes. The involvement of HARS in axon guidance highlights its participation in broader cellular functions, extending its significance beyond its canonical role in aminoacylation. HARS/HisRS Protein, Human (sf9, His) is the recombinant human-derived HARS/HisRS protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of HARS/HisRS Protein, Human (sf9, His) is 508 a.a., with molecular weight of ~59.6 kDa.

HARS, a pivotal enzyme, orchestrates the ATP-dependent ligation of histidine to the 3'-end of its corresponding tRNA by forming an aminoacyl-adenylate intermediate (His-AMP). This critical step in protein synthesis ensures the accurate incorporation of histidine into nascent polypeptide chains. Beyond its fundamental role in translation, HARS also emerges as a multifunctional player, contributing to axon guidance processes. The involvement of HARS in axon guidance highlights its participation in broader cellular functions, extending its significance beyond its canonical role in aminoacylation.

Human

Sf9 insect cells

N-His

P12081 (A2-C509)

3035  [NCBI]

Approximately 59.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.