GUCY2C/Guanylyl cyclase C Protein, Rat (HEK293, Myc, His)

GUCY2C, a guanylyl cyclase, crucially catalyzes cGMP synthesis from GTP.As a receptor for E.coli heat-stable enterotoxin, GUCY2C significantly stimulates cGMP accumulation in mammalian cells, emphasizing its role in mediating responses to specific external stimuli and showcasing intricate regulatory mechanisms in cellular signaling.GUCY2C/Guanylyl cyclase C Protein, Rat (HEK293, Myc, His) is the recombinant rat-derived GUCY2C/Guanylyl cyclase C protein, expressed by HEK293 , with N-His, C-Myc labeled tag.

GUCY2C, a guanylyl cyclase, plays a pivotal role in catalyzing the synthesis of cyclic (cGMP) from GTP. Acting as a receptor for the E. coli heat-stable enterotoxin, GUCY2C responds to the toxin by significantly stimulating the accumulation of cin mammalian cells expressing this protein. This interaction highlights the intricate regulatory mechanisms involved in cellular signaling and emphasizes the role of GUCY2C in mediating responses to specific external stimuli.

1.This product does not contain protein kinase domain.
2.Immobilized Rat GUCY2C at 5 μg/mL (100 μL/well) can bind Anti-GUCY2C Antibody. The ED₅₀ for this effect is 0.4595 ng/mL.

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  Immobilized Rat GUCY2C at 5 μg/mL (100 μL/well) can bind Anti-GUCY2C Antibody. The ED₅₀ for this effect is 0.4595 ng/mL.

Rat

HEK293

N-His;C-Myc

P23897 (S23-Q429)

25711  [NCBI]

Extracellular Domain

SQVRQKCHNGTYEISVLMMDNSAYKEPLQNLRDAVEEGLDIVRKRLREAELNVTVNATFIYSDGLIHKSGDCRSSTCEGLDLLREITRDRKMGCVLMGPSCTYSTFQMYLDTELNYPMISAGSFGLSCDYKETLTRILPPARKLMYFLVDFWKVNNAPFKTFSWNSSYVYKNGSEPEDCFWYLNALEAGVSYFSEVLSFKDVLRRSEQFQEILMGRNRKSNVIVMCGTPETFYNVKGDLKVADDTVVILVDLFSNHYFEDDTRAPEYMDNVLVLTLPPEKFIANASVSGRFPSERSDFSLAYLEGTLLFGHMLQTFLENGESVTTPKFARAFRNLTFQGLEGPVTLDDSGDIDNIMCLLYVSLDTRKYKVLMAYDTHKNQTIPVATSPNFIWKNHRLPNDVPGLGPQ

Approximately 70-90 kDa

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  ≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized after extensive dialysis against solution in PBS, 6-8% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.