HGFR Protein, Cynomolgus/Rhesus Macaque (HEK293)

HGFR Protein, a receptor tyrosine kinase, transduces signals by binding hepatocyte growth factor/HGF ligand. It regulates proliferation, scattering, morphogenesis, and cell survival. Upon ligand binding, HGFR undergoes autophosphorylation, creating docking sites for downstream molecules like PI3K, PLCG1, SRC, GRB2, STAT3, or GAB1, activating RAS-ERK, PI3K-AKT, and PLCgamma-PKC cascades. HGFR Protein, Cynomolgus/Rhesus Macaque (HEK293) is the recombinant Rhesus Macaque, cynomolgus-derived HGFR protein, expressed by HEK293 , with tag free.

Hepatocyte growth factor receptor (HGFR), a receptor tyrosine kinase, functions as a signal transducer from the extracellular matrix by binding to hepatocyte growth factor/HGF ligand. It plays a pivotal role in regulating diverse physiological processes, including proliferation, scattering, morphogenesis, and cell survival. Upon ligand binding at the cell surface, HGFR undergoes autophosphorylation on its intracellular domain, creating docking sites for downstream signaling molecules. Upon activation by ligand, it interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3, or the adapter GAB1, leading to the activation of multiple signaling cascades, including RAS-ERK, PI3 kinase-AKT, and PLCgamma-PKC. RAS-ERK activation is associated with morphogenetic effects, while PI3K/AKT coordinates prosurvival effects. In embryonic development, HGFR signaling contributes to gastrulation, the development and migration of neuronal precursors, angiogenesis, and kidney formation. During skeletal muscle development, it is crucial for the migration of muscle progenitor cells and the proliferation of secondary myoblasts. In adults, it participates in wound healing, organ regeneration, tissue remodeling, and promotes the differentiation and proliferation of hematopoietic cells. Additionally, in the context of microbial infection, HGFR acts as a receptor for Listeria monocytogenes internalin InlB, mediating the entry of the pathogen into cells^[1][2][3].

1.This product does not contain protein kinase domain.
2.Immobilized Cynomolgus HGF R at 2 μg/mL (100 μL/well) can bind Human HGF (HY-P72766). The ED₅₀ for this effect is 0.2285 μg/mL.

Rhesus Macaque; Cynomolgus

HEK293

Tag Free

XP_005550635.4 (E44-T951)=NP_001162100.2 (E25-T932)

102123512/704562  [NCBI]

Partial

ECKEALAKSEMNVNMKYQLPNFTAETAIQNVILHEHHIFLGATNYIYVLNEEDLQKVAEYKTGPVLEHPDCFPCQDCSSKANLSGGVWKDNINMALVVDTYYDDQLISCGSVNRGTCQRHVFPHNHTADIQSEVHCIFSPQIEEPNQCPDCVVSALGAKVLSSVKDRFINFFVGNTINSSYFPHHPLHSISVRRLKETKDGFMFLTDQSYIDVLPEFRDSYPIKYIHAFESNNFIYFLTVQRETLNAQTFHTRIIRFCSLNSGLHSYMEMPLECILTEKRKKRSTKKEVFNILQAAYVSKPGAQLARQIGASLNDDILFGVFAQSKPDSAEPMDRSAMCAFPIKYVNDFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEARRDEYRAEFTTALQRVDLFMGQFSEVLLTSISTFVKGDLTIANLGTSEGRFMQVVVSRSGPSTPHVNFLLDSHPVSPEVIVEHPLNQNGYTLVVTGKKITKIPLNGLGCRHFQSCSQCLSAPPFVQCGWCHDKCVRSEECPSGTWTQQICLPAIYKVFPTSAPLEGGTRLTICGWDFGFRRNNKFDLKKTRVLLGNESCTLTLSESTMNTLKCTVGPAMNKHFNMSIIISNGHGTTQYSTFSYVDPIITSISPKYGPMAGGTLLTLTGNYLNSGNSRHISIGGKTCTLKSVSNSILECYTPAQTISTEFAVKLKIDLANRETSIFSYREDPIVYEIHPTKSFISGGSTITGVGKNLHSVSVPRMVINVHEAGRNFTVACQHRSNSEIICCTTPSLQQLNLQLPLKTKAFFMLDGILSKYFDLIYVHNPVFKPFEKPVMISMGNENVLEIKGNDIDPEAVKGEVLKVGNKSCENIHLHSEAVLCTVPNDLLKLNSELNIEWKQAISSTVLGKVIVQPDQNFT

Approximately 40-50 & 75-90 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 6.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.