gp140 Protein, HIV-1 (AAC97548, HEK293, Fc)

gp140 is a non-cleaved exodomain of (gp160)3, with both its transmembrane segment (TM) and cytoplasmic tail (CT) truncated, and is often considered a possible replacement for natural Env spikes.However, most HIV-1 gp140 preparations are unstable and have an uneven conformation.gp140 Protein, HIV-1 (AAC97548, HEK293, Fc) is the recombinant Virus-derived gp140 protein, expressed by HEK293 , with C-hFc labeled tag.

HIV-1 envelope spike trimer (gp160)3, cleaved to (gp120/gp41)3, is a type I membrane protein that fuses the virus and host cell membrane to initiate viral infection. gp140 is a non-cleaved exodomain of (gp160)3, with both its transmembrane segment (TM) and cytoplasmic tail (CT) truncated, and is often considered a possible replacement for natural Env spikes. However, most HIV-1 gp140 preparations are unstable and have an uneven conformation^[1].

Virus

HEK293

C-hFc

AAC97548.1 (L33-K659)

/

Partial

LWVTVYYGVPIWKDANTTLFCASDAKAYDTEVHNVWATHACVPTDPSPQELKMENVTEEFNMWKNNMVEQMHTDIISLWDQSLKPCVQLTPLCVTLDCSYNITNNITNSITNSSVNMREEIKNCSFNMTTELRDKNRKVYSLFYKLDVVQINNGNNSSNLYRLINCNTSALTQARPKVTFEPIPIHYCAPAGYAILKCNDKEFNGTGLCKNVSTVQCTHGIRPVVSTQLLLNGSLAEGKVMIRSENITNNVKNIIVQLNESVTINCTRPNNNTRRSVRIGPGQTFYATGDIIGDIRQAHCNVSGSQWNKTLHQVVEQLRKYWNNNTIIFNSSSGGDLEITTHSFNCAGEFFYCNTSGLFNSTWVNGTTSSTSNGTITLPCRIKQIINMWQRVGQAMYAPPIQGVIKCESNITGLILTRDGGVNSSDSETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKARRRVVEREKRAVTLGAVFIGFLGTAGSTMGAASITLTVQARKLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICPTNVPWNSSWSNKSLDEIWENMTWLQWDKEISNYTIKIYELIEESQIQQERNEKDLLELDK

Approximately 97.4 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.