2. Recombinant Proteins
  3. Viral Proteins
  4. HIV Proteins
  5. HIV-1 gp140 Proteins
  6. gp140 Protein, HIV-1 (627a.a, HEK293, Fc)

gp140 Protein, HIV-1 (627a.a, HEK293, Fc)

Cat. No.: HY-P74884
COA Handling Instructions

gp140 is a non-cleaved exodomain of (gp160)3, with both its transmembrane segment (TM) and cytoplasmic tail (CT) truncated, and is often considered a possible replacement for natural Env spikes. However, most HIV-1 gp140 preparations are unstable and have an uneven conformation. gp140 Protein, HIV-1 (627a.a, HEK293, Fc) is the recombinant Virus-derived gp140 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of gp140 Protein, HIV-1 (627a.a, HEK293, Fc) is 627 a.a., with molecular weight of ~97.4 kDa.

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Description

gp140 is a non-cleaved exodomain of (gp160)3, with both its transmembrane segment (TM) and cytoplasmic tail (CT) truncated, and is often considered a possible replacement for natural Env spikes. However, most HIV-1 gp140 preparations are unstable and have an uneven conformation. gp140 Protein, HIV-1 (627a.a, HEK293, Fc) is the recombinant Virus-derived gp140 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of gp140 Protein, HIV-1 (627a.a, HEK293, Fc) is 627 a.a., with molecular weight of ~97.4 kDa.
Background

HIV-1 包膜突刺 三聚体(gp160)3, cleaved to (gp120/gp41)3 是一种 I 型膜蛋白，它融合病毒和宿主细胞膜，引发病毒感染。gp140 是 (gp160)3 的非切割外结构域，其跨膜段 (TM) 和细胞质尾部 (CT) 都被截断，通常被认为是天然 Env 尖峰的可能替代物。然而，大多数 HIV-1 gp140 制剂不稳定且构象不均匀。
Species

Virus
Source

HEK293
Tag

C-hFc
Accession

AAC97548.1 (L33-K659)
Gene ID

/
Molecular Construction
N-term
gp140 (L33-K659)
Accession # AAC97548.1
hFc
C-term
Synonyms
HIV-1 gp140 Protein (group M, subtype A, strain 92UG037.1) (Fc)
AA Sequence

LWVTVYYGVPIWKDANTTLFCASDAKAYDTEVHNVWATHACVPTDPSPQELKMENVTEEFNMWKNNMVEQMHTDIISLWDQSLKPCVQLTPLCVTLDCSYNITNNITNSITNSSVNMREEIKNCSFNMTTELRDKNRKVYSLFYKLDVVQINNGNNSSNLYRLINCNTSALTQARPKVTFEPIPIHYCAPAGYAILKCNDKEFNGTGLCKNVSTVQCTHGIRPVVSTQLLLNGSLAEGKVMIRSENITNNVKNIIVQLNESVTINCTRPNNNTRRSVRIGPGQTFYATGDIIGDIRQAHCNVSGSQWNKTLHQVVEQLRKYWNNNTIIFNSSSGGDLEITTHSFNCAGEFFYCNTSGLFNSTWVNGTTSSTSNGTITLPCRIKQIINMWQRVGQAMYAPPIQGVIKCESNITGLILTRDGGVNSSDSETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKARRRVVEREKRAVTLGAVFIGFLGTAGSTMGAASITLTVQARKLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICPTNVPWNSSWSNKSLDEIWENMTWLQWDKEISNYTIKIYELIEESQIQQERNEKDLLELDK

Molecular Weight

Approximately 97.4 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.
Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Shipping

Room temperature in continental US; may vary elsewhere.
Documentation

COA (220 KB)

Handling Instructions (2659 KB)

gp140 Protein, HIV-1 (627a.a, HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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