HIV-1 gp140 Protein (646a.a, HEK293, Fc)

HIV-1 gp140 Protein is the gp160 ectodomain, and can lead to the production of trimers that can mimic the native Env spike. HIV-1 gp140 trimers is able to elicit neutralizing antibody responses in immunized animals^[1][2]. HIV-1 gp140 Protein (646a.a, HEK293, Fc) is the recombinant Virus-derived HIV-1 gp140 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of HIV-1 gp140 Protein (646a.a, HEK293, Fc) is 646 a.a., with molecular weight of ~99.9 KDa.

HIV-1 gp140 Protein trimers is the uncleaved ectodomains of (gp160)₃, gp140, has been used as a surrogate for the native state of the cleaved, viral spike. The HIV-1 gp140 trimer has nearly all of the antigenic properties expected for native viral spikes. HIV-1 gp140 Protein can be used for research of vaccine development^[1]. HIV-1 gp140 trimers is able to elicit neutralizing antibody responses in immunized animals^[2].

Virus

HEK293

C-hFc

AAB61124.1 (N32-I677)

/

NLWVTVYYGVPVWKEAKTTLFCASDAKAYDAEVHNVWATHACVPTDPNPQEMVLENVTENFNMWENDMVEQMHQDIISLWDQSLKPCVKLTPLCVTLHCSNRTIDYNNRTDNMGGEIKNCSFNMTTEVRDKREKVHALFYRLDIVPLKNESSNTSGDYRLINCNTSAITQACPKVSFDPIPIHYCAPAGYAILKCNNKTFNGTGPCNNVSTIQCTHGTKPVVSTQLLLNGSLAEEEIIIRSKNLTDNVKTIIVHLNESVEINCTRPNNNTRKSIRIGPGQAFYATGEIIGDIRQAHCNISRTAWNKTLQEVGKKLAEHFPNKAIKFAKHSGGDLEITTHSFNCRGEFFYCNTSSLFNSTYTPNSTENITGTENSIITIPCRIKQIINMWQGVGRAMYAPPIEGILTCRSNITGLLLTRDGGTGMHDTEIFRPEGGDMRDNWRSELYKYKVVEIKPLGIAPTKAKRRVVEREKRAVGIGAVFLGFLGAAGSTMGAASITLTVQVRQLLSGIVQQQSNLLRAIEAQQHMLQLTVWGIKQLQTRVLAIERYLRDQQLLGIWGCSGKLICTTAVPWNSSWSNRSQEDIWNNMTWMQWDREISNYTNTIYRLLEDSQNQQEKNEQDLLALDKWQNLWTWFGITNWLWYIKI

Approximately 99.9 kDa.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.