HK3/Hexokinase-3 Protein, Human (sf9, His-GST)

The HK3 (hexokinase-3) protein promotes the phosphorylation of hexoses (i.e., D-glucose and D-fructose) to form hexose-6-phosphate (specifically, D-glucose 6-phosphate and 6-phosphate), Plays a vital role in cell metabolism. D-fructose 6-phosphate. HK3/Hexokinase-3 Protein, Human (sf9, His-GST) is the recombinant human-derived HK3/Hexokinase-3 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

The HK3 protein, also known as Hexokinase-3, plays a crucial role in glycolysis by catalyzing the phosphorylation of hexoses, including D-glucose and D-fructose, to form hexose 6-phosphates such as D-glucose 6-phosphate and D-fructose 6-phosphate, respectively. This enzyme mediates the initial and rate-limiting step of glycolysis, facilitating the conversion of D-glucose to D-glucose 6-phosphate. Through this enzymatic activity, HK3 contributes to the entry of glucose into the glycolytic pathway, a fundamental process in cellular energy metabolism. The phosphorylation of hexoses by HK3 is essential for subsequent metabolic pathways and energy production within the cell.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-His;N-GST

P52790 (M1-V923)

3101  [NCBI]

Full Length

MDSIGSSGLRQGEETLSCSEEGLPGPSDSSELVQECLQQFKVTRAQLQQIQASLLGSMEQALRGQASPAPAVRMLPTYVGSTPHGTEQGDFVVLELGATGASLRVLWVTLTGIEGHRVEPRSQEFVIPQEVMLGAGQQLFDFAAHCLSEFLDAQPVNKQGLQLGFSFSFPCHQTGLDRSTLISWTKGFRCSGVEGQDVVQLLRDAIRRQGAYNIDVVAVVNDTVGTMMGCEPGVRPCEVGLVVDTGTNACYMEEARHVAVLDEDRGRVCVSVEWGSFSDDGALGPVLTTFDHTLDHESLNPGAQRFEKMIGGLYLGELVRLVLAHLARCGVLFGGCTSPALLSQGSILLEHVAEMEDPSTGAARVHAILQDLGLSPGASDVELVQHVCAAVCTRAAQLCAAALAAVLSCLQHSREQQTLQVAVATGGRVCERHPRFCSVLQGTVMLLAPECDVSLIPSVDGGGRGVAMVTAVAARLAAHRRLLEETLAPFRLNHDQLAAVQAQMRKAMAKGLRGEASSLRMLPTFVRATPDGSERGDFLALDLGGTNFRVLLVRVTTGVQITSEIYSIPETVAQGSGQQLFDHIVDCIVDFQQKQGLSGQSLPLGFTFSFPCRQLGLDQGILLNWTKGFKASDCEGQDVVSLLREAITRRQAVELNVVAIVNDTVGTMMSCGYEDPRCEIGLIVGTGTNACYMEELRNVAGVPGDSGRMCINMEWGAFGDDGSLAMLSTRFDASVDQASINPGKQRFEKMISGMYLGEIVRHILLHLTSLGVLFRGQQIQRLQTRDIFKTKFLSEIESDSLALRQVRAILEDLGLPLTSDDALMVLEVCQAVSQRAAQLCGAGVAAVVEKIRENRGLEELAVSVGVDGTLYKLHPRFSSLVAATVRELAPRCVVTFLQSEDGSGKGAALVTAVACRLAQLTRV

Approximately 115 kDa,based on SDS-PAGE under reducing conditions.

≥ 85%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM Tris, 100 mM Nacl, pH 7.4, 25% glycerol, 0.5 mM PMSF, 0.1 mM EDTA, 0.5 mM GSH.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.