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  3. HLA-E*0103 Complex Protein, Human (Biotinylated, HEK293, His-Avi)

HLA-E*0103 Complex Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P77759
Handling Instructions

The HLA-E*0103 Complex, a non-classical major histocompatibility class Ib molecule, is crucial for immune self-nonself discrimination. It selectively binds VL9 peptides from classical MHC class Ia molecules, forming a complex with B2M. This complex acts as a ligand for KLRD1-KLRC1 on NK cells, facilitating immune monitoring. Under stress, HLA-E*0103 binds stress-induced chaperone peptides and HLA-G peptides, influencing NK cell activation and contributing to adaptive NK cell functions during pregnancy. Additionally, it presents pathogen-derived peptides to CD8-positive T cells, triggering an antimicrobial immune response. HLA-E*0103 Complex Protein, Human (Biotinylated, HEK293, His-Avi) is a recombinant protein dimer complex containing HLA-E*0103 and B2M/Beta-2-microglobulin Protein, expressed by HEK293 , with C-His, C-Avi labeled tag and VMAPRTLVL peptide. HLA-E*0103 Complex Protein, Human (Biotinylated, HEK293, His-Avi), has molecular weight of 52-60 kDa.

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Description

The HLA-E*0103 Complex, a non-classical major histocompatibility class Ib molecule, is crucial for immune self-nonself discrimination. It selectively binds VL9 peptides from classical MHC class Ia molecules, forming a complex with B2M. This complex acts as a ligand for KLRD1-KLRC1 on NK cells, facilitating immune monitoring. Under stress, HLA-E*0103 binds stress-induced chaperone peptides and HLA-G peptides, influencing NK cell activation and contributing to adaptive NK cell functions during pregnancy. Additionally, it presents pathogen-derived peptides to CD8-positive T cells, triggering an antimicrobial immune response. HLA-E*0103 Complex Protein, Human (Biotinylated, HEK293, His-Avi) is a recombinant protein dimer complex containing HLA-E*0103 and B2M/Beta-2-microglobulin Protein, expressed by HEK293 , with C-His, C-Avi labeled tag and VMAPRTLVL peptide. HLA-E*0103 Complex Protein, Human (Biotinylated, HEK293, His-Avi), has molecular weight of 52-60 kDa.

Background

HLA-E*0103 Complex, a non-classical major histocompatibility class Ib molecule, plays a pivotal role in immune self-nonself discrimination. This molecule forms a complex with B2M/beta-2-microglobulin and selectively binds nonamer self-peptides derived from the signal sequence of classical MHC class Ia molecules, specifically VL9 peptides (VMAPRT[V/L][L/V/I/F]L). The peptide-bound HLA-E-B2M heterotrimeric complex serves as a ligand for the inhibitory receptor KLRD1-KLRC1 on natural killer (NK) cells, enabling these cells to monitor the expression of other MHC class I molecules in healthy cells and tolerate self. During cellular stress, HLA-E*0103 preferentially binds signal sequence-derived peptides from stress-induced chaperones, resulting in impaired protection from NK cells. Moreover, it binds signal sequence-derived peptides from non-classical MHC class Ib HLA-G molecules, acting as a ligand for the NK cell activating receptor KLRD1-KLRC2, potentially contributing to adaptive NK cell functions and maternal-fetal tolerance during pregnancy. Additionally, HLA-E*0103 can bind and present pathogen-derived peptides to alpha-beta T cell receptors on unconventional CD8-positive cytotoxic T cells, triggering an antimicrobial immune response. This molecule also presents specific peptides from viruses like human cytomegalovirus, facilitating immune tolerance to infected cells.

Species

Human

Source

HEK293

Tag

C-His;C-Avi

Accession

P13747 (G25-I305)&P61769 (I21-M119)&VMAPRTLVL

Gene ID

3133  [NCBI]&567  [NCBI]

Synonyms
HLAE; sHLA-E; HLAE; MHC class I antigen E; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC; QA1
Molecular Weight

52-60 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

HLA-E*0103 Complex Protein, Human (Biotinylated, HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HLA-E*0103 Complex Protein, Human (Biotinylated, HEK293, His-Avi)
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HY-P77759
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