HNRNPR Protein, Human (sf9, His-GST)

hnRNP-R Protein, a constituent of ribonucleosomes, participates in precursor mRNA processing within the nucleus. It's part of the spliceosome C complex and involved in IGF2BP1-dependent mRNP granule complexes containing untranslated mRNAs. Additionally, hnRNP-R interacts with GTPBP1. HNRNPR Protein, Human (sf9, His-GST) is the recombinant human-derived hnRNP-R protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

The hnRNP-R Protein functions as a component of ribonucleosomes, integral complexes composed of at least 20 diverse heterogeneous nuclear ribonucleoproteins (hnRNP). Operating within the nucleus, hnRNP-R and other hnRNPs play a crucial role in the processing of precursor mRNA. Notably, hnRNP-R has been identified in the spliceosome C complex and is also present in an IGF2BP1-dependent mRNP granule complex, which encompasses untranslated mRNAs. Additionally, hnRNP-R engages in interactions with GTPBP1, further contributing to its involvement in dynamic cellular processes and highlighting its multifaceted role in RNA metabolism and regulation.

Human

Sf9 insect cells

N-GST;N-10*His

O43390-2 (A2-K636)

10236  [NCBI]

Full Length of Isoform-2

ANQVNGNAVQLKEEEEPMDTSSVTHTEHYKTLIEAGLPQKVAERLDEIFQTGLVAYVDLDERAIDALREFNEEGALSVLQQFKESDLSHVQNKSAFLCGVMKTYRQREKQGSKVQESTKGPDEAKIKALLERTGYTLDVTTGQRKYGGPPPDSVYSGVQPGIGTEVFVGKIPRDLYEDELVPLFEKAGPIWDLRLMMDPLSGQNRGYAFITFCGKEAAQEAVKLCDSYEIRPGKHLGVCISVANNRLFVGSIPKNKTKENILEEFSKVTGLTEGLVDVILYHQPDDKKKNRGFCFLEYEDHKSAAQARRRLMSGKVKVWGNVVTVEWADPVEEPDPEVMAKVKVLFVRNLATTVTEEILEKSFSEFGKLERVKKLKDYAFVHFEDRGAAVKAMDEMNGKEIEGEEIEIVLAKPPDKKRKERQAARQASRSTAYEDYYYHPPPRMPPPIRGRGRGGGRGGYGYPPDYYGYEDYYDDYYGYDYHDYRGGYEDPYYGYDDGYAVRGRGGGRGGRGAPPPPRGRGAPPPRGRAGYSQRGAPLGPPRGSRGGRGGPAQQQRGRGSRGSRGNRGGNVGGKRKADGYNQPDSKRRQTNNQQNWGSQPIAQQPLQQGGDYSGNYGYNNDNQEFYQDTYGQQWK

Approximately 98 kDa, based on SDS-PAGE under reducing conditions.

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 500 mM NaCl, 1 mM TCEP, 0.5 mM PMSF, 10% Glycerol, 2 mM GSH, 0.1 mM EDTA, pH 8.5, 8% Trehalose.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.