HNRNPH1 Protein, Human (His-SUMO)
Based on 1 Customer Validation
The HNRNPH1 protein is an important component of the hnRNP complex and plays a key role in pre-mRNA processing events leading to mRNA conversion in the cytoplasm. Functionally, it cooperates with CUGBP1 to regulate alternative splicing of pre-mRNA, specifically inhibiting the inclusion of insulin receptor (IR) pre-mRNA exon 11 in myoblasts. HNRNPH1 Protein, Human (His-SUMO) is the recombinant human-derived HNRNPH1 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The HNRNPH1 protein is an important component of the hnRNP complex and plays a key role in pre-mRNA processing events leading to mRNA conversion in the cytoplasm. Functionally, it cooperates with CUGBP1 to regulate alternative splicing of pre-mRNA, specifically inhibiting the inclusion of insulin receptor (IR) pre-mRNA exon 11 in myoblasts. HNRNPH1 Protein, Human (His-SUMO) is the recombinant human-derived HNRNPH1 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.
Background
The HNRNPH1 protein plays a crucial role as a component of heterogeneous nuclear ribonucleoprotein (hnRNP) complexes, pivotal entities in the processing events that precede the functional transformation of pre-mRNAs into translatable mRNAs in the cytoplasm. Functionally, HNRNPH1 mediates the regulation of pre-mRNA alternative splicing, with notable inhibitory effects on insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblasts, a process it accomplishes in collaboration with CUGBP1. HNRNPH1 demonstrates a specific binding affinity for IR RNA and poly(RG), and it forms part of a ternary complex alongside FUBP2, PTBP1, and PTBP2. Additionally, HNRNPH1 is identified in the spliceosome C complex and interacts with IGF2BP1, CUGBP1 (in an RNA-dependent manner), and MBNL1 (in an RNA-independent manner). These interactions underscore the multifaceted and dynamic role of HNRNPH1 in orchestrating pre-mRNA processing and alternative splicing, contributing to the intricate regulation of gene expression.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His;N-SUMO
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Accession
P31943 (M2-A449)
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Molecular Construction
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N-term
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6*His-SUMO
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HNRNPH1 (M2-A449)
Accession # P31943 -
C-term
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Protein Length
Full Length
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Synonyms
HNRNPH1; Heterogeneous Nuclear Ribonucleoprotein H1 (H); Prev. HNRPH1; HNRPH; Heterogeneous Nuclear Ribonucleoprotein H; HnRNP H; HnRNPH; NEDCDS; Heterogeneous Nuclear Ribonucleoprotein H1; Epididymis Secretory Sperm Binding Protein
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AA Sequence
MLGTEGGEGFVVKVRGLPWSCSADEVQRFFSDCKIQNGAQGIRFIYTREGRPSGEAFVELESEDEVKLALKKDRETMGHRYVEVFKSNNVEMDWVLKHTGPNSPDTANDGFVRLRGLPFGCSKEEIVQFFSGLEIVPNGITLPVDFQGRSTGEAFVQFASQEIAEKALKKHKERIGHRYIEIFKSSRAEVRTHYDPPRKLMAMQRPGPYDRPGAGRGYNSIGRGAGFERMRRGAYGGGYGGYDDYNGYNDGYGFGSDRFGRDLNYCFSGMSDHRYGDGGSTFQSTTGHCVHMRGLPYRATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEDAVAAMSKDKANMQHRYVELFLNSTAGASGGAYEHRYVELFLNSTAGASGGAYGSQMMGGMGLSNQSSYGGPASQQLSGGYGGGYGGQSSMSGYDQVLQENSSDFQSNIA
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Predicted Molecular Mass
65.1 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.2 μm solution of PBS, 6% Trehalose, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US;may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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